Toxicity and Dermatokinetic Analysis of Ibrutinib in Human Skin Models.

Ibrutinib (IBR) is a tyrosine kinase inhibitor under investigation in preclinical and clinical settings as an alternative treatment for melanoma. Nevertheless, the limited oral bioavailability of IBR and the need for high doses of the drug to kill melanoma cells are major drawbacks for this purpose. Considering that melanoma is restricted to the skin at early stages, the topical application of IBR might constitute an effective and safer administration route.

Histologic and proteomic profile of two methods to decellularize human dental pulp tissue.

Objective: The present study compared the structural and proteomic architecture of extracellular matrices (ECM) of decellularized human dental pulp using two previously described protocols.

Design: Pulp tissue from 150 molars was extracted and three treatments took place, based on the Matoug-Elwerfelli Group (MG) and the Song Group (SG) protocols and an untreated pulp group (CG), to examine histoarchitecture and the effectiveness of the decellularization process, using histological analysis (n = 12) and scanning electron microscopy (SEM) (n = 3). Protein extraction took place using 100 mg dry weight of pulp, in triplicates for each group, and the shotgun proteome analysis was performed by nanoUPLC-MS.

Combination of selol nanocapsules and magnetic hyperthermia hinders breast tumor growth in aged mice after a short-time treatment.

Short time treatment with reduced dosages of selol-loaded PLGA nanocapsules (NcSel) combined with magnetic hyperthermia (MHT) is evaluated in aged Erhlich tumor-bearing mice. Clinical, hematological, biochemical, genotoxic and histopathological parameters are assessed during 7 d treatment with NcSel and MHT, separately or combined. The time evolution of the tumor volume is successfully modeled using the logistic mathematical model.

Human papillomavirus (HPV) genotype distribution in penile carcinoma: Association with clinic pathological factors.

Background: Penile carcinoma (PC) is a rare, highly mutilating disease, common in developing countries. The evolution of penile cancer includes at least two independent carcinogenic pathways, related or unrelated to HPV infection.

Objectives: To estimate the prevalence, identify HPV genotypes, and correlate with clinicopathological data on penile cancer.

Evaluation of HLA-G, HLA-E, and PD-L1 proteins in oral osteosarcomas.

Objective: The aim of this study was to investigate the expression of human leukocyte antigens (HLAs) G and E and programmed death-ligand 1 (PD-L1) in oral osteosarcoma (OO) (n = 13). The relationship between the expression of these molecules and histologic grading and metastasis was also evaluated.

Study Design: HLA-G, HLA-E, and PD-L1 were identified by immunohistochemistry.

The relation between FoxP3⁺ regulatory T cells and fungal density in oral paracoccidioidomycosis: a preliminary study.

Regulatory T (Treg) cells may play an important role in the pathogenesis of paracoccidioidomycosis (PCM), but data on the role of Treg cells in the context of oral PCM are still scarce. The objectives of this study were to investigate the density of FoxP3(+) T regulatory cells in oral PCM and to correlate the results with the density of Paracoccidioides brasiliensis in the lesions. Cases of chronic oral PCM seen between 2000 and 2008 were included in this study.

Salivary gland tumor: a review of 599 cases in a Brazilian population.

Salivary gland tumors consist of a group of heterogeneous lesions with complex clinicopathological characteristics and distinct biological behaviors. Worldwide series show a contrast in the relative incidence of salivary gland tumors, with some discrepancies in clinicopathological data. The main aim of this study was to describe demographic characteristics of 599 cases in a population from Central Brazil over a 10-year period and compare these with other epidemiological studies.

Genetic polymorphisms of carcinogen metabolizing enzymes are associated with oral leukoplakia development and p53 overexpression.

Background: Genetic polymorphisms of carcinogen-metabolizing enzyme genes have been associated with the risk of oral squamous cell carcinoma and oral leukoplakia. The overexpression of p53 protein is the most common genetic alteration in head and neck cancer. In the present study the combined or isolated presence of glutathione S-transferases GSTM1, GSTT1, GSTP1, and the cytochrome P450 oxidases CYP1A1 and CYP2E1 polymorphisms and oral leukoplakia development in a Brazilian sample of individuals was investigated, together with the effect of these polymorphisms on p53 overexpression in the lesions.

GSTM1 polymorphism and oral leukoplakia.

Background: Molecular epidemiological studies have now provided evidence that an individual susceptibility to cancer is mediated by genetic and environmental factors. Genetic polymorphisms have been described for enzymes involved in the metabolism of tobacco carcinogens and cancer risk is determined by the degree of expression and/or activity of enzymes involved in carcinogen activation or deactivation. The objective of this study was to investigate the GSTM1 null polymorphism and the risk for oral leukoplakia in individuals with tobacco-smoking habit in a Brazilian population.

GSTT1 polymorphism and oral leukoplakia.

Background: Considering that tobacco is the main etiological factor in oral cancer and that oral leukoplakia is the most prevalent potentially malignant lesion, together with the fact that GSTT1 polymorphism increases the risk of oral squamous cell carcinoma, the purpose of this study was to investigate the GSTT1 null polymorphism and the risk of oral leukoplakia in individuals with tobacco smoking habits in a Brazilian population.

Materials And Methods: The GSTT1 genotypes of 72 tobacco smoking patients with oral leukoplakia and 72 tobacco smoking healthy subjects were studied by PCR-based methods.

Results: The frequency of the GSTT1 null genotype in the group with oral leukoplakia (48.

Odontogenic tumors: a study of 340 cases in a Brazilian population.

Background: The aim of this study was to determinate the relative frequency of odontogenic tumors (OTs) in a Brazilian population and to compare this data with previous reports.

Methods: We reviewed the achieves of 19 123 specimens from oral pathology laboratory of Universidade Federal de Minas Gerais, from 1954 to 2004. Using the criteria of histologic typification published by the World Health Organization in 1992, we classified the OTs.

Primary syphilis of oral mucosa: case report of an unusual manifestation.

Dental clinicians and other health care providers have long been concerned about a variety of infectious agents that may be transmitted within the dental setting. Many infectious diseases, including human immunodeficiency virus, hepatitis, tuberculosis, and syphilis are important both because of their potential transmissibility and because the first manifestations of the disease may appear in the oral cavity. Oral disease as a consequence of primary syphilis is rare.