Application of HPCCC Combined with Polymeric Resins and HPLC for the Separation of Cyclic Lipopeptides Muscotoxins A⁻C and Their Antimicrobial Activity.

Muscotoxins are cyanobacterial cyclic lipopeptides with potential applications in biomedicine and biotechnology. In this study, CCALA125 strain extracts were enriched by polymeric resin treatment, and subjected to HPCCC affording three cyclic lipopeptides (⁻), which were further repurified by semi-preparative HPLC, affording , , and , with a purity of 86%, 92%, and 90%, respectively. The chemical identities of ⁻ were determined as muscotoxins A and B, respectively, by comparison with previously reported ESI-HRMS/MS data, whereas was determined as a novel muscotoxin variant (muscotoxin C) using NMR and ESI-HRMS/MS data.