Immunoaffinity clean-up and direct fluorescence measurement of aflatoxins B1 and M1 in pig liver: comparison with high-performance liquid chromatography determination.

An improved analytical method for aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) determination in pig liver is described, using an aqueous methanol extraction, an immunoaffinity column clean-up step and a direct fluorometric measurement for toxin detection and quantification. A detection limit of 1.0 microg kg-1 was achieved for AFB1 and AFM1.