Publications by authors named "Elisabeth Falkenstein"

Progesterone has been shown to be a physiologically relevant inducer of the sperm acrosome reaction. A novel protein intrinsic to microsomal membranes, membrane progesterone receptor (mPR, now termed progesterone membrane receptor component 1, PGMRC1) that binds progesterone with high affinity has been cloned from porcine liver previously, and corresponding antibodies mitigate the progesterone induced acrosome reaction. In this study we aimed at the localization of mPR in porcine spermatozoa.

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Aldosterone can elicit rapid nongenomic effects both in vivo and in vitro, often mediated by signal transduction cascades. However, it is not understood how these rapid effects are initiated. In this study we show that aldosterone leads to rapid activation of mitogen activated protein kinases ERK1/2 in the cortical collecting duct cell line M-1.

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Article Synopsis
  • The study investigates how aldosterone, a hormone, affects the expression of various genes in human renal epithelial cells and suggests that there may be mechanisms beyond the usual receptor pathways involved in kidney and heart disease.
  • Researchers found that specific genes were significantly up-regulated after aldosterone treatment, indicating its impact on cellular processes, but some genes showed resistance to common receptor inhibitors.
  • The research highlights the need for further exploration of these non-classical regulatory mechanisms to better understand why aldosterone antagonists like spironolactone may not always be effective in treating kidney disease.
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Steroids may exert their action in living cells by several ways: 1). the well-known genomic pathway, involving hormone binding to cytosolic (classic) receptors and subsequent modulation of gene expression followed by protein synthesis. 2).

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Nongenomic action of aldosterone has been observed in many cell types which often are different from the classic target tissues for mineralocorticoid action, such as vascular cells. As judged from their time scale and insensitivity toward inhibitors of protein synthesis, effects are not mediated by the classic mineralocorticoid receptor pathway. Here we summarize studies on rapid in vitro aldosterone effects, e.

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