Publications by authors named "Elisabete A Almeida"
Article Synopsis
- Antimicrobial resistance is a major public health issue globally, particularly with non-typhoidal Salmonella, which the study investigates through 2,511 isolates collected from 2016 to 2023.
- The study identified 10 common serotypes, with Heidelberg, Typhimurium, and Enteritidis being the most prevalent, and found significant antimicrobial resistance, especially among the Heidelberg and Mbandaka serotypes from nonhuman sources.
- Resistance to several drugs was widespread, although Salmonella Typhi remained mostly susceptible, emphasizing the need for ongoing surveillance and effective public health monitoring.
Article Synopsis
- * Whole genome sequencing and antimicrobial susceptibility tests reveal that the isolate (288_18) is resistant to several antibiotics and carries a 67 kb transferable plasmid with the bla gene.
- * The findings stress the need for improved surveillance to control the spread of antibiotic resistance, particularly in clinically relevant strains like Salmonella Agona.
Article Synopsis
- Salmonellosis is a widely reported zoonotic disease linked to foodborne outbreaks, with continuous monitoring of Salmonella serotype distribution in Sao Paulo, Brazil, since the 1950s.
- A study analyzed 9,014 Salmonella isolates from 2004 to 2020, revealing 39.4% from humans and 60.6% from nonhuman sources, with a notable presence in animals, food, and their environments.
- The research highlighted 104 serotypes in human samples, with Enteritidis showing a decline, while nonhuman isolates included 156 serotypes, showing both commonalities and trends, indicating various reservoirs for potential human salmonellosis.
Genomic characterization of a multi-drug resistant, CTX-M-65-producing clinical isolate of Salmonella Infantis isolated in Brazil.
Microbes Infect
August 2022
Article Synopsis
- - A patient in Brazil was found to have a multi-drug resistant Salmonella Infantis that produces CTX-M-65.
- - Whole genome sequencing revealed that the antibiotic resistance gene (bla) is located on a pESI-like megaplasmid in this particular strain (ST32).
- - Phylogenetic analysis showed that this Brazilian isolate is closely related to similar IncFIB Salmonella Infantis strains found in food and poultry in the USA.
Most known plasmids are identified by conferring virulence or antimicrobial resistance phenotypes and such characteristics aid in the success of the dispersion of different plasmid types between bacteria from different sources. This study aimed to perform the subtyping of the plasmid-mediated quinolone resistance, detected in Salmonella spp. A total of 34 Salmonella strains non-susceptible to ciprofloxacin were evaluated.
View Article and Find Full Text PDFBackground: The primary method of molecular subtyping for the identification and investigation of outbreaks has been pulsed-field gel electrophoresis (PFGE). In some cases, this technique has not been able to show discrimination between the unrelated strains that can be achieved by whole genome sequencing (WGS).
Methods: The aim of this study was to determine the strengths and drawbacks of WGS using different analytic approaches compared to traditional typing method, PFGE, for retrospectively typing clusters cases of 28 S.
Article Synopsis
- - A total of thirty-eight Salmonella isolates were collected from various points in the peanut supply chain across three Brazilian states (São Paulo, Minas Gerais, Bahia) and analyzed for antimicrobial resistance and virulence genes using PFGE and other tests.
- - All isolates showed resistance to sulfonamide, with only one strain demonstrating additional resistance to ampicillin, while intermediary resistance was noted for kanamycin, streptomycin, and ceftazidime.
- - Six virulence genes were found in all isolates, with a distinct distribution of pulsotypes revealed: pulsotype 1 was exclusive to Minas Gerais and pulsotype 7 was identified in both São Paulo and Bahia, indicating limited multidrug resistance overall.
We describe the draft genome sequence of the clinical strain 03_7315, isolated in 2016 from the blood of a diabetic patient who died of septicemia after ingestion of seafood. The draft genome, with 4,755,588 bp covering two chromosomes, presented 4,434 genes, 4,213 coding sequences, and 117 pseudogenes.
View Article and Find Full Text PDFBackground: Tuberculosis is a major health problem in São Paulo, Brazil, which is the most populous and one of the most cosmopolitan cities in South America. To characterize the genetic diversity of Mycobacterium tuberculosis in the population of this city, the genotyping techniques of spoligotyping and MIRU were applied to 93 isolates collected in two consecutive years from 93 different tuberculosis patients residing in São Paulo city and attending the Clemente Ferreira Institute (the reference clinic for the treatment of tuberculosis).
Findings: Spoligotyping generated 53 different spoligotype patterns.
In order to study certain epidemiological features of multidrug-resistant tuberculosis (MDR-TB) carriers and their influence on the control and treatment, a group of patients was evaluated over a four-year period, selected by: Mycobacterium tuberculosis isolation from sputum; resistance to Rifampin, Isoniazid and one more drug, or, failure of reserve regimen, all cases were from a tuberculosis reference unit in the City of S o Paulo. A total of 182 patients were reviewed, with a mean age of 35.7 +/- 6.
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