Publications by authors named "Elisa C S Santos"
Article Synopsis
- Offspring can be produced from porcine oocytes that were vitrified at the germinal vesicle (GV) stage, but the success rate of embryo development is low.
- The study compared nuclear morphology, cumulus expansion, transzonal projections, and biochemical levels between vitrified cumulus-oocyte complexes (COCs) and control groups, discovering that vitrification causes premature nuclear maturation while cytoplasmic maturation remains mostly unaffected.
- Findings suggest that reduced embryo development could be due to early nuclear maturation, leading to aging of oocytes, and indicate a need for further research to understand the underlying mechanisms of these effects during vitrification.
Staining with brilliant cresyl blue (BCB) may be used for oocyte selection, but BCB staining itself and the most commonly used selection medium (DMPBS) may compromise the development of porcine oocytes in vitro. This study evaluated DNA fragmentation, nuclear maturation, the area of migration of cortical granules (CG) and embryo development for stained (BCB+) and unstained (BCB-) oocytes incubated in DMPBS and in a modified medium (ReproPel) tested for the first time. Unexposed (UN), BCB+ and BCB- oocytes were incubated composing six groups: DMPBS/UN; DMPBS/BCB+; DMPBS/BCB-; ReproPel/UN; ReproPel/BCB+; and ReproPel/BCB-.
View Article and Find Full Text PDFIn vitro assays for vesper mice (Calomys laucha) sperm using heterologous substrates from nonrodent species.
J Exp Zool A Ecol Genet Physiol
February 2012
Small vesper mice (Calomys laucha) may be considered as an animal model for in vitro fertilization studies, but limited data about in vitro evaluations of their sperm quality and fertility are available. The in vitro penetration (IVP) assay is used to estimate potential sperm fertility for many mammal species, but it still requires reduction in cost and labor. This study tested improvements in the IVP assay for C.
View Article and Find Full Text PDF