Plasmid DNA Production in Proteome-Reduced .

The design of optimal cell factories requires engineering resource allocation for maximizing product synthesis. A recently developed method to maximize the saving in cell resources released 0.5% of the proteome of by deleting only three transcription factors.

Enhanced plasmid DNA production by enzyme-controlled glucose release and an engineered Escherichia coli.

Objectives: To evaluate the combination of a culture medium employing glucoamylase-mediated glucose reléase from a gluco-polysaccharide and an E. coli strain engineered in its glucose transport system for improving plasmid DNA (pDNA) production.

Results: The production of pDNA was tested using E.