Publications by authors named "Eling Goh"
Hidden sequence specificity in loading of single-stranded RNAs onto Drosophila Argonautes.
Nucleic Acids Res
April 2019
Article Synopsis
- Argonaute proteins help control genes by using small RNA guides, and they might be pickier than we thought when choosing which RNA to bind to.
- A new method called HISSA lets scientists study which RNA sequences Argonautes prefer by testing many different ones at once.
- In experiments with fruit flies, they found that one type of Argonaute (AGO2) likes G-rich RNA sequences, while another (AGO1) prefers a specific 'GAC' sequence, which could help in designing treatments using RNA to silence genes.
Article Synopsis
- Scientists have come up with new ways to study how RNA-binding proteins (RBPs) interact with RNA and found that some proteins, thought to be random, actually have specific preferences for certain RNA sequences.
- They discovered that Argonaute (AGO) proteins, which usually bind to small RNA strands, also prefer specific sequences, not just certain bases.
- To study these preferences, researchers created a special method that helps analyze many different RNA sequences at once, helping us understand how AGO proteins choose which RNA to bind.
Here we present a detailed analysis of the alternative splicing regulation of human CD46, which generates different isoforms with distinct functions. CD46 is a ubiquitous membrane protein that protects host cells from complement and plays other roles in immunity, autophagy, and cell adhesion. CD46 deficiency causes an autoimmune disorder, and this protein is also involved in pathogen infection and cancer.
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