Characterization of Caco-2 cell monolayer drug transport properties by cassette dosing using UV/fluorescence HPLC.

Cassette dosing is commonly used in pharmacokinetic studies to decrease time, labor and expenditures. Cassette dosing (having several compounds in a mixture) has been used to screen in vitro permeabilities in Caco-2 cell monolayers. The cassette dosing method has accelerated both transport experiments and sample analyses, which are both part of Caco-2 permeability screening.