Asymmetric correlation: a noise robust similarity measure for template matching.

We present an efficient and noise robust template matching method based on asymmetric correlation (ASC). The ASC similarity function is invariant to affine illumination changes and robust to extreme noise. It correlates the given non-normalized template with a normalized version of each image window in the frequency domain.

Identification and comparative analysis of telomerase RNAs from Candida species reveal conservation of functional elements.

The RNA component of telomerase (telomerase RNA; TER) varies substantially both in sequence composition and size (from approximately 150 nucleotides [nt] to >1500 nt) across species. This dramatic divergence has hampered the identification of TER genes and a large-scale comparative analysis of TER sequences and structures among distantly related species. To identify by phylogenetic analysis conserved sequences and structural features of TER that are of general importance, it is essential to obtain TER sequences from evolutionarily distant groups of species, providing enough conservation within each group and enough variation among the groups.

A critical three-way junction is conserved in budding yeast and vertebrate telomerase RNAs.

The telomerase ribonucleoprotein copies a short template within its integral RNA moiety onto eukaryotic chromosome ends, compensating for incomplete replication and degradation. Non-template regions of telomerase RNA (TER) are also crucial for telomerase function, yet they are highly divergent in sequence among species and their roles are largely unclear. Using both phylogenetic and mutational analyses, we predicted secondary structures for TERs from Kluyveromyces budding yeast species.