Residual levels of pesticides in filet green beans from Souss-Massa, Morocco.

A total of 30 samples of filet green beans (Phaseolus vulgaris) from various popular markets in the Souss-Massa region (Morocco) were analyzed during 2021 for the presence of pesticide residues. We performed a QuEChERS extraction (Quick, Easy, Cheap, Effective, Rugged, and Safe) combined with Liquid Chromatography-Mass Spectrometry (LC-MS/MS) and Gas Chromatography-Mass Spectrometry (GC-MS/MS). The method was validated in accordance with SANTE 11312/2021 guidelines.

Pesticide contamination pattern from Morocco, insights into the surveillance situation and health risk assessment: a review.

The widespread application of pesticides in Morocco's agriculture renders their monitoring in food and environmental samples very necessary. Recent years have witnessed a growing interest in reporting studies related to the monitoring of pesticide residues in food, water, groundwater, and soil as well as their quantitative health risk assessment. Most published studies have been done by university researchers.

Monitoring 432 potential pesticides in tomatoes produced and commercialized in Souss Massa region-Morocco, using LC-MS/MS and GC-MS/MS.

In this work, we monitored 432 pesticide residues in 39 tomato samples (cherry tomato) obtained from local markets in Souss Massa region-Morocco. We used a QuEChERS combined with LC-MS/MS and GC-MS/MS, and the method was validated based on SANTE 11312/2021 guideline. The limits of quantification (LOQ) obtained for most of the pesticides analyzed are at the limit with default EU MRLs or well below other EU MRLs.

Modified-QuEChERS-LC/MS method for the analysis of aflatoxins and ochratoxin A in coriander seeds.

Coriander seeds consumption, like other spices, has increased due to its culinary use, medicinal benefits, and its essential oil. However, there are few reports in the scientific literature on the prevalence of mycotoxins in this spice. In this report, an easy and simple method was proposed for the determination of the aflatoxin group (AFB1, AFB2, AFG1, and AFG2) and ochratoxin A (OTA) in coriander seeds.

A simple extraction method with no lipid removal for the determination of aflatoxins in almonds by liquid chromatography tandem-mass spectrometry (LC-MS/MS).

The present study describes a simple and rapid method for the determination of aflatoxins in almonds using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Aflatoxins were extracted using a modified QuEChERS method with little sample preparation, excluding the use of laborious purification procedures. Extracts were frozen overnight to separate the majority of lipids.

Chemometric Approach Based on Accuracy Profile and Data Chronological Distribution as a Tool to Detect Performance Degradation and Improve the Analytical Quality Control for Aflatoxins' Analysis in Almonds Using UPLC-MS/MS.

One of the main objectives of routine laboratories is the development of simple and reliable methods as well as meeting fit-for-purpose criteria for regulatory surveillance. In this study, the accuracy profiles and the evaluation of the distribution of results in the case of aflatoxins in almonds have been performed using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The method consists of designing the experiment and using certified reference material (CRM) to evaluate the bias, to calculate the combined uncertainty, and to construct the control charts.

A facile extraction method followed by UPLC-MS/MS for the analysis of aflatoxins and ochratoxin A in raw coffee beans.

A UPLC-MS/MS method was developed and validated for the determination of aflatoxin B (AFB1), aflatoxin B (AFB2), aflatoxin G (AFG1), aflatoxin G (AFG2) and ochratoxin A (OTA) in raw coffee samples. Mycotoxins were extracted using a modified QuEChERS method with little sample preparation excluding clean-up and enrichment procedures. Linearity was demonstrated for the five mycotoxins in the range 0.

Mycotoxins in food: a review on liquid chromatographic methods coupled to mass spectrometry and their experimental designs.

The development of a multi-mycotoxins method using LC-MS/MS is necessary and it is clear that the development of such method involves many compromises in the choice of the different parameters. This review summarizes applications using conventional experimental designs and some recent studies using response surface methodology (RSM) as a mathematical modeling tool for the optimization of extraction procedures. The authors also discuss pros and cons of the different procedures.

Optimization and validation of a liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the determination of aflatoxins in maize.

A LC-MS/MS method has been optimized and validated for the determination of aflatoxins (AFB1, AFB2, AFG1 and AFG2) in maize. Extraction was performed using a modified QuEChERS method with little sample preparation without the need for purification procedure. Determination was performed by high pressure liquid chromatography (HPLC) coupled to tandem mass spectrometry (MS/MS).

Molecularly imprinted polymer cartridges coupled to high performance liquid chromatography (HPLC-UV) for simple and rapid analysis of fenthion in olive oil.

A combination of molecular modelling and a screening of the library of non-imprinted polymers (NIPs) was used to identify acrylamide as a functional monomer with high affinity towards fenthion, organophosphate insecticide, which is frequently used in the treatment of olives. A good correlation was found between the screening tests and modelling of monomer-template interactions performed using a computational approach. Acrylamide-based molecularly imprinted polymer (MIP) and non-imprinted polymer (NIP) were thermally synthesised in dimethyl formamide (porogen) using ethylene glycol dimethacrylate as a cross-linker and 1,1-azo-bis (isobutyronitrile) as an initiator.

Computational and experimental investigation of molecular imprinted polymers for selective extraction of dimethoate and its metabolite omethoate from olive oil.

This work presents the development of molecularly imprinted polymers (MIPs) for the selective extraction of dimethoate from olive oil. Computational simulations allowed selecting itaconic acid as the monomer showing the highest affinity towards dimethoate. Experimental validation confirmed modelling predictions and showed that the polymer based on IA as functional monomer and omethoate as template molecule displays the highest selectivity for the structurally similar pesticides dimethoate, omethoate and monocrotophos.

Acetylcholinesterase immobilized on magnetic beads for pesticides detection: application to olive oil analysis.

This work presents the development of bioassays and biosensors for the detection of insecticides widely used in the treatment of olive trees. The systems are based on the covalent immobilisation of acetylcholinesterase on magnetic microbeads using either colorimetry or amperometry as detection technique. The magnetic beads were immobilised on screen-printed electrodes or microtitration plates and tested using standard solutions and real samples.

Molecular imprinting solid phase extraction for selective detection of methidathion in olive oil.

A specific adsorbent for extraction of methidathion from olive oil was developed. The design of the molecularly imprinted polymer (MIP) was based on the results of the computational screening of the library of polymerisable functional monomers. MIP was prepared by thermal polymerisation using N,N'-methylene bisacrylamide (MBAA) as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as a cross-linker.