The importance of Real-Time Polymerase Chain Reaction method in diagnosis of intestinal parasites in cases with diarrhea.

Diarrheal diseases cause significant morbidity in developing countries, and have a potential to cause severe complications. Stool samples collected from 272 patients with diarrhea in Adana, Turkey were screened using different laboratory methods such as the wet mount examination (WME), modified trichrome stain (MTS), modified acid-fast (MAF) stain, enzyme linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (realtime PCR) for intestinal parasites. Intestinal parasites were detected in 25.

Feconomics®; a new and more convenient method, the routine diagnosis of intestinal parasitic infections.

Direct wet mount examination and concentration are the most commonly used methods for detecting intestinal parasites from fecal samples. Concentration methods are used when there are fewer protozoan cyst, coccidian oocyst, microsporidial spore, helminth egg, and larvae in the fecal samples. Early detection of the causative intestinal parasites plays a significant role in implementing timely and correct treatment, which relieves the patients' symptoms and also prevents recurrences.

Investigation of Cryptosporidium spp. antigen by ELISA method in stool specimens obtained from patients with diarrhea.

Cryptosporidium spp. is an important parasitic protozoan causing diarrhea in developing and developed countries. The agent causes severe life-threatening diarrhea especially in immunocompromised hosts.

Identification of Blastocystis hominis isolates from asymptomatic and symptomatic patients by PCR.

Despite years of study, the pathogenic role of Blastocytis hominis is still controversial. Genotypic differences between the asymptomatic and symptomatic isolates should assist in determining the pathogenicity of Blastocystis. In this study, we genotyped 32 Blastocystis isolates obtained from 12 asymptomatic healthy individuals and 20 symptomatic patients pain by polymerase chain reaction using known seven kinds of sequence tagged site primers in this study.