Extension of Maximal Lifespan and High Bone Marrow Chimerism After Nonmyeloablative Syngeneic Transplantation of Bone Marrow From Young to Old Mice.

The goal of this work was to determine the effect of nonablative syngeneic transplantation of young bone marrow (BM) to laboratory animals (mice) of advanced age upon maximum duration of their lifespan. To do this, transplantation of 100 million nucleated cells from BM of young syngeneic donors to an old nonablated animal was performed at the time when half of the population had already died. As a result, the maximum lifespan (MLS) increased by 28 ± 5%, and the survival time from the beginning of the experiment increased 2.

Informational Theory of Aging: The Life Extension Method Based on the Bone Marrow Transplantation.

The method of lifespan extension that is a practical application of the informational theory of aging is proposed. In this theory, the degradation (error accumulation) of the genetic information in cells is considered a main cause of aging. According to it, our method is based on the transplantation of genetically identical (or similar) stem cells with the lower number of genomic errors to the old recipients.

Numerical Matrices Method for nonlinear system identification and description of dynamics of biochemical reaction networks.

A flexible Numerical Matrices Method (NMM) for nonlinear system identification has been developed based on a description of the dynamics of the system in terms of kinetic complexes. A set of related methods are presented that include increasing amounts of prior information about the reaction network structure, resulting in increased accuracy of the reconstructed rate constants. The NMM is based on an analytical least squares solution for a set of linear equations to determine the rate parameters.