Antonie Van Leeuwenhoek
December 2024
Four salt-tolerant and aromatics degrading strains used in this study were isolated from polluted technogenic soil on the territory of the Verkhnekamsk potash deposit (Russia). The strains were aerobic, Gram-stain-positive, non-motile, non-endospore-forming irregular rods, exhibiting a marked rod-coccus growth cycle. They contained lysine-based peptidoglycan, teichulosonic acid and poly(glycosyl phosphate) polymers in the cell walls.
View Article and Find Full Text PDFPuromycin (Puro) is a natural aminonucleoside antibiotic that inhibits protein synthesis by its incorporation into elongating peptide chains. The unique mechanism of Puro finds diverse applications in molecular biology, including the selection of genetically engineered cell lines, in situ protein synthesis monitoring, and studying ribosome functions. However, the key step of Puro biosynthesis remains enigmatic.
View Article and Find Full Text PDFDuring a cultural diversity survey on hydrolytic bacteria in saline alkaline soils, a hydrolytic actinobacterium strain ACPA39 was enriched and isolated in pure culture from a soda solonchak soil in southwestern Siberia. It forms a substrate mycelium with rod-shaped sporangia containing 1-3 exospores. The isolate is obligately alkaliphilic, growing at pH 7.
View Article and Find Full Text PDFAntimicrobial resistance is a global public health threat. One of the possible ways to solve this problem is phage therapy, but the instability of bacteriophages hinders the development of this approach. A bacteriophage delivery system that stabilizes the phage is one of the possible solutions to this problem.
View Article and Find Full Text PDFAn intracellular bacterium, strain IAS, was observed to infect several species of the plant-parasitic nematode genus (, , , , and ). The bacterium could not be recovered on axenic medium. The 16S rRNA gene sequence of IAS was found to be new, being related to the family Burkholderiaceae, class Betaproteobacteria.
View Article and Find Full Text PDFHere, we report a draft genome sequence of the strain Coralloluteibacterium stylophorae LMG 29479, acquired from the Belgian Coordinated Collections of Microorganisms. The genus currently includes only one species with a validly published name. These genome sequencing data are important for the phylogeny of the family.
View Article and Find Full Text PDFA haloalkaliphilic hydrolytic actinobacterium, strain ACPA22, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia. The isolate was facultatively alkaliphilic, growing at pH 6.5-10.
View Article and Find Full Text PDFPhage therapy is a great alternative to antibiotic drugs, but it can't effectively overcome the over-acidic medium of the stomach. We offer the use of polyelectrolyte microcapsules as a protective means of bacteriophage. It is necessary to understand the influence of polyelectrolytes on bacteriophage survival.
View Article and Find Full Text PDFA draft genome sequence of the bacterial isolate Alg18-2.2, recovered from the highly saline and alkaline lake Gudzhirganskoe (Buryatia, Russia), was determined. The results of bacterial identification using 16S rRNA gene sequence and whole-genome analyses suggest that the bacterium belongs to a novel genus.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
September 2018
A facultative anaerobic, rod-shaped, endospore-forming and non-motile bacterium was isolated from permafrost sediment cores in the Kolyma lowland, Siberia, Russia. The permafrost isolate clustered with members of the genus Cohnella on the basis of 16S rRNA gene sequence analysis and showed the highest sequence similarity to Cohnella saccharovorans CJ22 (96.3 %), followed by Cohnella cellulosilytica FCN3-3 (96.
View Article and Find Full Text PDFA draft genome sequence of sp. strain VKM Ac-2630 was derived using Ion Torrent sequencing technology. The genome size of this strain is 3.
View Article and Find Full Text PDFA draft genome sequence of "Cohnella kolymensis" strain B-2846 was derived using IonTorrent sequencing technology. The size of the assembly and G+C content were in agreement with those of other species of this genus. Characterization of the genome of a novel species of Cohnella will assist in bacterial systematics.
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