Background: Ca and HO are second messengers that regulate a wide range of cellular events in response to different environmental and developmental cues. In plants, stress-induced HO has been shown to initiate characteristic Ca signatures; however, a clear picture of the molecular connection between HO-induced Ca signals and HO-induced cellular responses is missing, particularly in cereal crops such as barley. Here, we employed RNA-seq analyses to identify transcriptome changes in roots and leaves of barley after HO treatment under conditions that inhibited the formation of cytosolic Ca transients.
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