Assessment of Distribution and Diversity of Pyrrolizidine Alkaloids in the Most Prevalent Boraginaceae Species in Macedonia.

Systematic study of extraction efficiency of pyrrolizidine alkaloids (PAs) and corresponding pyrrolizidine alkaloid N-oxides (PANOs) from plant material for subsequent LC/MS analysis was carried out. The optimal extraction was achieved with methanol and one clean up step using SPE C18 column. With the optimized LC-ESI-MS/MS method using ion trap, the distribution and diversity of PAs and PANOs in plant material (leaves, flowers and stems) obtained from wild-growing E.