Publications by authors named "Elena Markina"
The development of osteopenia is one of the most noticeable manifestations of the adverse effects of space factors on crew members. The Hippo signaling pathway has been shown to play a central role in regulating the functional activity of cells through their response to mechanical stimuli. In the present study, the components of the Hippo pathway and the protective properties of osteodifferentiation inducers were investigated under simulated microgravity (smg) using a heterotypic bone marrow cell culture model, which allows for the maintenance of the close interaction between the stromal and hematopoietic compartments, present in vivo and of great importance for both the fate of osteoprogenitors and hematopoiesis.
View Article and Find Full Text PDFMuscle and skeleton structures are considered most susceptible to negative factors of spaceflights, namely microgravity. Three-dimensional clinorotation is a ground-based simulation of microgravity. It provides an opportunity to elucidate the effects of microgravity at the cellular level.
View Article and Find Full Text PDFRodent hindlimb unloading (HU) model was developed to elucidate responses/mechanisms of adverse consequences of space weightlessness. Multipotent mesenchymal stromal cells (MMSCs) were isolated from rat femur and tibia bone marrows and examined ex vivo after 2 weeks of HU and subsequent 2 weeks of restoration of load (HU + RL). In both bones, decrease of fibroblast colony forming units (CFU-f) after HU with restoration after HU + RL detected.
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- Bone and muscle tissues are highly affected by various forms of low gravity, particularly simulated microgravity (sμg), leading to significant changes in bone marrow (BM) in an experimental mouse model.
- Male C57Bl/6N mice were categorized into three groups for a study that assessed how hindlimb suspension (HS) and subsequent short-term reloading (RL) affected the quantity and functionality of BM mononucleated cells (MNCs), as well as their hematopoietic and stromal progenitors.
- Results showed that HS caused a significant reduction in MNCs and disrupted their proliferation and differentiation, while RL partially restored stromal precursor activity but failed to completely recover hematopoietic cell functions.
Elucidation of the spaceflight (SF) effects on the adult stem and progenitor cells is an important goal in space biology and medicine. A unique opportunity for this was provided by project "BION-M1". The purpose of this study was to evaluate the effects of 30-day SF on biosatellite, 7-day recovery (SFR), and subsequent ground control (GC) experiment on the mononuclear cells (MNCs) from C57/BI/6N murine tibia bone marrow.
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