Publications by authors named "Elena Leontyevskaya"
Article Synopsis
- This study examined the antimicrobial properties of a specific strain, revealing that its culture fluid contains agents effective against various bacteria.
- The strain was found to produce outer membrane vesicles that exhibit bacteriolytic effects on both Gram-positive and Gram-negative bacteria.
- Transcriptomic analysis highlighted numerous genes related to bacteriolytic enzymes and secondary metabolites with antimicrobial potential, emphasizing the importance of isolating and researching these antimicrobial agents.
The aim of the study was to search for, isolate and characterize new bacteriolytic enzymes that show promising potential for their use in medicine, agriculture and veterinary. Using a transcriptomic analysis, we annotated in VKM B-2533 the genes of known bacteriolytic and antifungal enzymes, as well as of antibiotics, whose expression levels increased when cultivated on media conducive to the production of antimicrobial agents. The genes of the secreted putative bacteriolytic proteases were also annotated.
View Article and Find Full Text PDFArticle Synopsis
- The crystal structure of VKM B-2533 β-lytic protease (Blp) was successfully determined, identifying its similarity to the M23 protease family.
- Comparisons between Blp and the LasA structural homologue showed differences in the amino acids of their active site grooves, despite no variations in optimal reaction conditions for their bacteriolytic activities.
- Blp demonstrates a wider range of effectiveness against various target cells, suggesting that the geometry and charge of amino acids in the active site groove influence its ability to break down different types of peptidoglycan.
Article Synopsis
- Lysobacter capsici VKM B-2533 and Lysobacter gummosus 10.1.1 are identified as potentially valuable strains for developing new antimicrobial drugs.* -
- The whole-genome sequences of these strains were sequenced, with total lengths of 6,239,188 base pairs and 6,056,609 base pairs, respectively.* -
- Sequencing was performed using advanced technologies, specifically the Illumina and Nanopore platforms.*
A successful homologous expression system based on VKM B-2533 and the plasmid pBBR1-MCS5 was first developed for a promising bacteriolytic enzyme of this bacterium, β-lytic protease (Blp). In the expression strains, gene expression under the regulation of the GroEL(A) and T5 promoters increased by 247- and 667-fold, respectively, as compared with the wild-type strain. After the cultivation of the expression strains P- and P-, the Blp yield increased by 6.
View Article and Find Full Text PDFOuter membrane vesicles (OMVs) produced by Gram-negative bacteria constitute important factors in defining interactions with the extracellular milieu. sp. XL1 produces OMVs capable of lysing microbial cells due to the presence in their cargo of bacteriolytic protease L5 (AlpB).
View Article and Find Full Text PDFDraft Genome Sequence of the Type Strain Lysobacter capsici VKM B-2533.
Microbiol Resour Announc
January 2021
VKM B-2533 is a promising strain for isolation of new lytic agents. Here, we report a draft genome sequence of this strain, consisting of 131 scaffolds with a total length of 6,196,943 bp. The results obtained will aid in the discovery and study of biologically active compounds important for biomedicine.
View Article and Find Full Text PDF