Dynamic holography in bacteriorhodopsin/gelatin films: effects of light-dark adaptation at different humidity.

This work examines the kinetics of dynamic holography responses in light-adapted and dark-adapted bacteriorhodopsin (BR) films at different humidity. We have demonstrated that the kinetics of the diffraction efficiency in wild type BR films is quite different in dark-adapted and light-adapted samples. The holographic recording kinetics, which depends on the duration of incubation in the dark after light adaptation at different humidity values, was studied in depth.

14-Fluoro-bacteriorhodopsin gelatin films for dynamic holography recording.

The first dynamic holography recording using 14-fluoro-(14-F) bacteriorhodopsin (BR) gelatin films has been achieved. 14-F BR is an artificial BR pigment made by reconstitution of bacterioopsin (native BR without chromophore) with synthetic 14-F retinal. Low-intensity red light from a cw He-Ne laser was used for dynamic holography recording on the 14-F wild type (WT) BR and 14-F D96N mutant BR in gelatin films.