Description of three new Pseudomonas species isolated from aquarium fish: Pseudomonas auratipiscis sp. nov., Pseudomonas carassii sp. nov. and Pseudomonas ulcerans sp. nov.

Pseudomonas species constitute a significant group of pathogens in aquarium fish and frequently cause haemorrhagic septicaemia. This study conducted a taxonomic characterization of Pseudomonas isolates from aquarium fish exhibiting deep ulceration and general disease signs. A polyphasic approach was employed to ascertain the taxonomic affiliation of the strains.

Stutzerimonas decontaminans sp. nov. isolated from marine polluted sediments.

Strains 19SMN4 and ST27MN3 were isolated from marine sediments after enrichment with 2-methylnaphthalene and were classified as Pseudomonas stutzeri genomovar 4. Four other strains, BG 2, HT20, HT24, and A7, were isolated from sulphide-oxidizing bioreactors or activated sludge affiliated with the same clade in the 16S rRNA phylogenetic tree. P.

Genome-Based Taxonomy of the Genus and Proposal of sp. nov. and sp. nov. and Emended Descriptions of and .

is a recently proposed genus within the comprising strains in the formerly phylogenetic group of . At least sixteen named species have to be included in the genus, together with 22 genomovars of . To clarify the taxonomy of , a core-genome phylogeny of 200 strains in the genus was inferred and monophyletic strains with average nucleotide identities (ANIb) with values equal to or higher than 95 were grouped in the same phylogenomic species.

Past, present and future of the boundaries of the Pseudomonas genus: Proposal of Stutzerimonas gen. Nov.

Pseudomonas is one the best studied bacterial genera, and it is the genus with the highest number of species among the gram-negative bacteria. Pseudomonas spp. are widely distributed and play relevant ecological roles; several species are commensal or pathogenic to humans, animals and plants.

Pseudomonas lalucatii sp. nov. isolated from Vallgornera, a karstic cave in Mallorca, Western Mediterranean.

Caves are extreme underground environments colonized by oligotrophic bacterial communities that influence mineral transformations. The identification at the species level is important and this study aims to the taxonomic characterisation of four bacterial strains previously isolated from rock surfaces and water samples from a karstic cave located on Mallorca (Spain) that were assigned to the genus Pseudomonas according to 16S rRNA nucleotide sequence analysis. Sequence analysis of the RNA polymerase sigma factor gene (rpoD) allocated these strains to the P.

Pseudomonas anatoliensis sp. nov and Pseudomonas iridis sp. nov. isolated from fish.

In a study carried out between 2013 and 2018 in fish farms in Turkey, several putative novel species were isolated. The 16S rRNA nucleotide sequences of fourteen strains of Gram-negative rods, which were isolated from asymptomatic and symptomatic rainbow trouts (Onchorhynchus mykiss), placed them under the genus Pseudomonas. The similarity values of the concatenated nucleotide sequences of the rpoD, rpoB, gyrB and 16S rRNA genes clustered these isolates into the P.

sp. nov., sp. nov. sp. nov. and sp. nov. isolated from rainbow trout.

Six Gram negative, motile bacteria were isolated from rainbow trout (). The 16S rRNA sequence similarity values grouped them in the (strains P49, P50, 154a and P154b), (strain P115) and (strain P155) phylogenetic subgroups in the genus . The DNA G+C content ranged from 58.

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia.

The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars.

Species Diversity Along the Danube River Assessed by Gene Sequence and MALDI-TOF MS Analyses of Cultivated Strains.

A collection of 611 isolated from 14 sampling sites along the Danube River were identified previously by MALDI-TOF MS with the VITEK MS system and were grouped in 53 clusters by their main protein profiles. The strains were identified in the present study at the phylospecies level by gene sequencing. Partial sequences of the gene of 190 isolates representatives of all clusters were analyzed.

High-quality draft genome sequences of DSM 14164, DSM 17497, DSM 15088, DSM 21245 and DSM 16006: taxonomic considerations.

is the bacterial genus of Gram-negative bacteria with the highest number of recognized species. It is divided phylogenetically into three lineages and at least 11 groups of species. The group of species is one of the most versatile and best studied.

Pseudomonas sivasensis sp. nov. isolated from farm fisheries in Turkey.

A study of 91 isolates from fish farms in Turkey showed that isolates P7, P11, P24b, P29, P72, P73 and P158 belonged to the genus Pseudomonas according to 16S rRNA nucleotide sequence analysis. The analysis of the sequences of the RNA polymerase sigma factor gene (rpoD) located these strains in the Pseudomonas fluorescens lineage of species within the P. fluorescens subgroup, close to the cluster composed of the species Pseudomonas grimontii, Pseudomonas marginalis and Pseudomonas panacis.

Genomics in Bacterial Taxonomy: Impact on the Genus .

The introduction of genomics is profoundly changing current bacterial taxonomy. Phylogenomics provides accurate methods for delineating species and allows us to infer the phylogeny of higher taxonomic ranks as well as those at the subspecies level. We present as a model the currently accepted taxonomy of the genus and how it can be modified when new taxonomic methodologies are applied.

sp. nov., isolated from clinical specimens.

Strains A31/70, CCUG 58779 and SD129 were Gram-stain-negative, short rods, motile by one polar flagellum and isolated from clinical specimens in Botswana, Sweden and Spain, respectively. The 16S rRNA sequence similarity values grouped them in the phylogenetic group of species. The DNA G+C content ranged from 65.

New species of pathogenic Pseudomonas isolated from citrus in Tunisia: Proposal of Pseudomonas kairouanensis sp. nov. and Pseudomonas nabeulensis sp. nov.

A collection of Pseudomonas strains was isolated in different regions of Tunisia in the period 2016-2017 from the fruits and leaves of Citrus sinensis cv. 'Valencia Late' and Citrus limon cv. 'Eureka' plants with symptoms of blast and black pit disease.

Pseudomonas gallaeciensis sp. nov., isolated from crude-oil-contaminated intertidal sand samples after the Prestige oil spill.

Strains V113, V92 and V120 have been isolated from sand samples taken at the Atlantic intertidal shore in Galicia, Spain, after the Prestige oil spill. A preliminary analysis of the 16S rRNA and the partial rpoD gene sequences indicated that these strains belonged to the Pseudomonas genus, but they were distinct from any known Pseudomonas species. They were extensively characterized by a polyphasic taxonomic approach and phylogenetic data that confirmed that these strains belonged to the Pseudomonas pertucinogena group.

Draft Genome Sequence of Pseudomonas oceani DSM 100277, a Deep-Sea Bacterium.

DSM 100277 was isolated from deep seawater in the Okinawa Trough at 1390 m. belongs to the group. Here, we report the draft genome sequence of , which has an estimated size of 4.

Clarification of Taxonomic Status within the Species Group Based on a Phylogenomic Analysis.

The phylogenetic group comprises 15 recognized bacterial species and more than 60 pathovars. The classification and identification of strains is relevant for practical reasons but also for understanding the epidemiology and ecology of this group of plant pathogenic bacteria. Genome-based taxonomic analyses have been introduced recently to clarify the taxonomy of the whole genus.

Draft Genome Sequence of the Marine Bacterium VGXO14.

The type strain of (VGXO14), isolated from a crude oil-polluted marine sand sample, is a member of the phylogenetic group. Here, we report the genome sequence (3.83 Mb) of to gain insights into the biology and taxonomy of marine spp.

Pseudomonas caspiana sp. nov., a citrus pathogen in the Pseudomonas syringae phylogenetic group.

In a screening by multilocus sequence analysis of Pseudomonas strains isolated from diverse origins, 4 phylogenetically closely related strains (FBF58, FBF102, FBF103, and FBF122) formed a well-defined cluster in the Pseudomonas syringae phylogenetic group. The strains were isolated from citrus orchards in northern Iran with disease symptoms in the leaves and stems and its pathogenicity against citrus plants was demonstrated. The whole genome of the type strain of the proposed new species (FBF102=CECT 9164=CCUG 69273) was sequenced and characterized.

Draft Genome Sequence of Strain CCUG 46540, a Deep-Sea Bacterium.

strain CCUG 46540 (KMM 330) was isolated from a deep-sea sponge specimen collected in the Philippine Sea at a depth of 750 m. The draft genome has an estimated size of 4.0 Mb, exhibits a G+C content of 61.