Publications by authors named "Elena Garcia Lorenzo"
Systemic sclerosis (SSc) is an autoimmune disorder with high morbidity and mortality, is difficult to diagnose early, and has no curative treatment. PSGL-1 is a leukocyte receptor whose deficiency in mice promotes an SSc-like disease. ADAM8, a metalloprotease that cleaves PSGL-1, is implicated in inflammatory processes.
View Article and Find Full Text PDFObjective: To analyze the effect of single nucleotide polymorphisms (SNPs) with well-known functional impact of methylenetetrahydrofolatereductase (MTHFR; rs1801131 and rs1801133), the membrane transporter ABCB1 (rs1045642), the AICAR transformylase/IMP cyclohydrolase (ATIC; rs2372536) and folyl-polyglutamatesynthetase (FPGS; rs1544105), on liver and bone marrow toxicity of methotrexate (MTX).
Patients And Methods: We analyzed 1415 visits from 350 patients of the PEARL (Princesa Early Arthritis Register Longitudinal) study: (732 with MTX, 683 without MTX). The different SNPs were genotyped using specific TaqMan probes (Applied Biosystems).
Article Synopsis
- Methotrexate (MTX) has distinct effects on macrophages involved in rheumatoid arthritis (RA), identified through its transcriptional influence on proinflammatory (GM-MØ) and anti-inflammatory (M-MØ) macrophage subtypes.
- Research methods included gene expression profiling and molecular pathway analysis to understand how MTX affects these macrophages.
- The study found that MTX primarily alters gene expression in proinflammatory GM-MØ, revealing that their sensitivity to MTX is linked to the expression of thymidylate synthase (TS) and the activity of p53, with CCL20 and LIF identified as potential markers for this responsiveness.