Determination of nitrofuran metabolites in honey using a new derivatization reagent, magnetic solid-phase extraction and LC-MS/MS.

In this study, 5-nitro-2-furaldehyde (5-NFA) was proposed as a new derivatizing agent for nitrofuran metabolites. It reacts with nitrofuran metabolites producing the parent nitrofurans (furazolidone, furaltadone, nitrofurantoin, and nitrofurazone). Magnetic hypercrosslinked polystyrene (HCP/FeO) was first used for magnetic solid phase extraction (MSPE) clean-up before the determination of nitrofuran metabolite derivatives in honey via LC-MS/MS.