Publications by authors named "Elena Coluccio"
Identifying activated T lymphocytes and differentiating antigen-specific from bystander T cells is crucial for understanding adaptive immune responses. This study investigates the efficacy of activation-induced markers (AIMs) in distinguishing these cell populations. We measured the expression of commonly used AIMs (CD25, CD38, CD40L, CD69, CD137, HLA-DR, ICOS, and OX40) in an in vitro T-cell activation system and evaluated their sensitivity, specificity, and positive predictive value.
View Article and Find Full Text PDFArticle Synopsis
- Many individuals infected with SARS-CoV-2 remain asymptomatic, underscoring the importance of serological testing to understand infection trends and immunity levels.
- A study of 8,798 blood donors in Milan from July 2020 to February 2021 revealed a rise in seroprevalence of anti-nucleoprotein and anti-spike antibodies from approximately 4% to 15% during the second wave of the virus.
- Seroconversion was influenced by factors like blood type (more in non-O types) and the timing of blood donations, while levels of anti-RBD antibodies showed a decline over time.
Anemia is a common feature of liver and bowel diseases. Although the main causes of anemia in these conditions are represented by gastrointestinal bleeding and iron deficiency, autoimmune hemolytic anemia should be considered in the differential diagnosis. Due to the epidemiological association, autoimmune hemolytic anemia should particularly be suspected in patients affected by inflammatory and autoimmune diseases, such as autoimmune or acute viral hepatitis, primary biliary cholangitis, and inflammatory bowel disease.
View Article and Find Full Text PDFBackground: Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion-associated mortality. Antibodies against human leucocyte antigens (HLA) and human neutrophil antigens (HNA) are often detected in the implicated donors. We investigated the incidence and aetiology of TRALI in Lombardy.
View Article and Find Full Text PDFObjectives: Transfusion-related acute lung injury (TRALI) is a rare but serious complication which can occur after transfusion of blood components. In this report we describe our flow-cytometry approach to the laboratory diagnosis of a case of TRALI in a recipient of fresh frozen plasma containing human leukocyte antigen (HLA) class II antibodies.
Methods: The post-transfusion reaction work-up included the direct and indirect Granulocyte Immunofluorescence Test (GIFT) on the recipient's neutrophils collected before and after the reaction and on the serum from the recipient and from all implicated donors; flow-cytometry bead-based screening and identification assay for HLA class I and II antibodies in donor sera and flow cytometry cross-matching on T and B patient's lymphocytes.
Background: Several drugs can cause immune hemolytic anemia. Here a patient who developed hemolytic anemia after treatment with teicoplanin is described.
Case Report: Owing to a two-vessel disease, a 68-year-old white man underwent coronary artery bypass grafting.