A novel bioelectrochemical interface based on in situ synthesis of gold nanostructures on electrode surfaces and surface activation by Meerwein's salt. A bioelectrochemical sensor for glucose determination.

A novel effective bioelectrochemical sensor interface for enzyme biosensors is proposed. The method is based on in situ synthesis of gold nanostructures (5-15 nm) on the thin-film electrode surface using the oleylamine (OA) method, which provides a high-density, stable, electrode interface nanoarchitecture. New method to activate the surface of the OA-stabilized nanostructured electrochemical interface for further functionalization with biomolecules (glucose oxidase enzyme) using Meerwein's salt is proposed.