Protocol for obtaining doubled haploids in isolated microspore culture for poorly responsive genotypes of brassicaceae family.

In this protocol for obtaining doubled haploids plants (DH), we propose a new method for microspore isolation. This method is useful for genotypes of the Brassicaceae family with low responsiveness to DH technology. For such crops, it allows increasing the embryo yield several times and sometimes obtaining embryos for the first time.

Embryogenesis of European Radish ( L. subsp. Convar. Radicula) in Culture of Isolated Microspores In Vitro.

The European radish is one of the most unresponsive crops in the family to embryogenesis in microspore culture. The aim of this work was to study the process of embryogenesis of European radish and its biological features. In this study, the embryogenesis of European radish is described in detail with illustrative data for the first time.