Numerical and experimental investigation of a droplet vitrification process: A thermofluidic analysis for cryopreservation.

One of the biggest challenges in studying vitrification protocols for small volumes of biological materials, especially the microdroplet vitrification protocol, is measuring the solidification rate, requiring equipment with a high level of technology, making it practically impossible to measure the degree of crystallization. An alternative is using mathematical models applied in computer simulations (CFD), helping to improve and develop new vitrification protocols. This study investigates the vitrification process utilizing the microdroplet method through experimental and numerical analysis.