A vector set for systematic metabolic engineering in Saccharomyces cerevisiae.

A set of shuttle vectors was constructed to facilitate expression of genes for metabolic engineering in Saccharomyces cerevisiae. Selectable markers include the URA3, TRP1, MET15, LEU2-d8, HIS3 and CAN1 genes. Differential expression of genes can be achieved as each marker is available on both CEN/ARS- and 2 µ-containing plasmids.

[Nursing teaching and the national curricular directives: utopia x reality].

This article aims at provoking reflections concerning Nursing education in the light of the Curricular Directives for the Undergraduate Course in Nursing and their relation to health policies and the current labor market. The facts reported show curricular changes in Nursing education in Brazil have historically been concerned with the adequacy of the nurse's formation to the interests of the labor market. However, the challenge in nurse's formation needs to overcome the focus of these interests and to effectively insert the future nurse into the health system, committed to the transformations demanded for the exercise of citizenship.

[Publications about nursing education in the Revista da Escola de Enfermagem da USP].

Scientific production in nursing education field provide important contributions to professional formation and nursing practice. Since we believe this perspective, we carried out a survey aimed at identifying and analyzing nursing education-related articles, building empiric categories from the collected data. This revision is a bibliographical, descriptive and quantitative survey using as source articles published in the Revista da Escola de Enfermagem da USP from 1967 to 2002.

Use of RT-PCR and DNA microarrays to characterize RNA recovered by non-invasive tape harvesting of normal and inflamed skin.

We describe a non-invasive approach for recovering RNA from the surface of skin via a simple tape stripping procedure that permits a direct quantitative and qualitative assessment of pathologic and physiologic biomarkers. Using semi-quantitative RT-PCR we show that tape-harvested RNA is comparable in quality and utility to RNA recovered by biopsy. It is likely that tape-harvested RNA is derived from epidermal cells residing close to the surface and includes adnexal structures and present data showing that tape and biopsy likely recover different cell populations.