Eco-friendly bupropion detection sensor with co-formulated dextromethorphan in AUVELITY tablet and spiked plasma.

Molecularly Imprinted Polymers (MIPs) are synthetic materials designed to selectively recognize and bind to specific target molecules. The process of determining Bupropion (BUP) using MIPs involves preparing the MIP, extracting the target molecule, and conducting subsequent analysis. A bio-inspired MIP-based electrochemical sensor was developed to detect BUP, utilizing the specific binding of MIPs to Bupropion molecules, enabling precise and sensitive detection.

High performance anion exchange chromatographic and colorimetric methods for quality assessment of total and free polysaccharide content in type b conjugate vaccine containing lactose.

The presence of lactose as a stabilizer in type b (Hib) conjugate vaccine is a challenge for chromatographic resolution of its total and free poly ribosyl ribitol phosphate (PRP) content. Sample pretreatment using ultrafiltration was performed and had removed ≥95% of lactose in shorter time compared to the conventional dialysis process. Separation of free unconjugated PRP was performed using solid-phase extraction C cartridges.

Molecular size distribution assessment of Haemophilus influenzae vaccine containing lactose by HPAEC-PAD and colorimetric assays.

Molecular size distribution of Haemophilus influenzae type b (Hib) conjugate vaccine is an important indicator for its immunogenicity and stability. Molecular size distribution was evaluated by High-Performance Protein Chromatography on Sepharose CL-4B column, and fractions were pooled. The use of high flow rate, incorporation of a calibration standard with the injected buffer and pooling method yielded a superior assay compared to conventional pharmacopeial method.

Validated Smart Different Chromatographic Methods for Selective Quantification of Acefylline Piperazine, Phenobarbital Sodium and Methylparaben Additive in Bulk and Pharmaceutical Dosage Form.

Two chromatographic methods have been proposed for the simultaneous determination of acefylline piperazine (ACEF) and phenobarbital (PHENO) in presence of methylparaben as additive in pharmaceutical dosage form. The first method was thin-layer chromatography. The separation was achieved using silica gel as stationary phase and chloroform: methanol: glacial acetic acid (2.

Facile imprinted polymer for label-free highly selective potentiometric sensing of proteins: case of recombinant human erythropoietin.

In the current study, a molecularly imprinted polymer (MIP)-based potentiometric sensor was fabricated for a label-free determination of recombinant human erythropoietin (rhEPO). The MIP sensor was operated under zero current conditions using tetra-butyl ammonium bromide as a marker ion. A highly ordered rhEPO surface imprinted layer was prepared using 3-aminopropyl triethoxysilane and tetraethoxysilane as a monomer and cross-linker, respectively, under mild reaction conditions.

Preparation and characterization of 96-well microplates coated with molecularly imprinted polymer for determination and biosimilarity assessment of recombinant human erythropoietin.

Synthesis and applications of molecularly imprinted polymers (MIP) are rapidly growing. In this study, a biomimetic MIP was prepared through silanes polymerization on the surface of 96-well microplates using recombinant human erythropoietin-alfa (rhEPO) as a template molecule. The rhEPO was immobilized onto the plate surface using bi-functional cross-linker and a thin imprinted layer following sol-gel procedure was constructed.

Preparation and characterization of pH-responsive polyacrylamide molecularly imprinted polymer: Application to isolation of recombinant and wild type human serum albumin from biological sources.

In this work pH-responsive neutral and cationic polyacrylamide molecularly imprinted polymers (nMIP and cMIP, respectively) were prepared for separation of recombinant and wild type human serum albumin (HSA, pI 4.7) using mixture of polymerization initiators. The effect of pH during preparation and adsorption stages at pI±2.

Simultaneous determination of piracetam and vincamine by spectrophotometric and high-performance liquid chromatographic methods.

A mixture of piracetam and vincamine was determined by 3 different methods. The first was the determination of piracetam and vincamine using the ratio-spectra first-derivative (DD1) spectrophotometric technique at 209 and 293 nm in concentration ranges of 10-45 and 2-14 microg/mL with mean recoveries of 99.22 +/- 0.

Application of derivative, derivative ratio, and multivariate spectral analysis and thin-layer chomatography-densitometry for determination of a ternary mixture containing drotaverine hydrochloride, caffeine, and paracetamol.

New selective, precise, and accurate methods are described for the determination of a ternary mixture containing drotaverine hydrochloride (I), caffeine (II), and paracetamol (III). The first method uses the first (D1) and third (D3) derivative spectrophotometry at 331 and 315 nm for the determination of (I) and (III), respectively, without interference from (II). The second method depends on the simultaneous use of the first derivative of the ratio spectra (DD1) with measurement at 312.

Development of membrane electrodes for the selective determination of hyoscine butylbromide.

Four polyvinyl chloride (PVC) membrane sensors for the determination of hyoscine butylbromide are described and characterized. The sensors are based on the use of the ion association complexes of hyoscine cation with ammonium reineckate counter anions as ion exchange sites in the PVC matrix. The membranes incorporate ion association complexes of hyoscine with dibutylsebathete (sensor 1), dioctylphthalate (sensor 2), nitrophenyl octyl ether (sensor 3) and beta-cyclodextrin (sensor 4).

Simultaneous determination of hyoscine butylbromide and ketoprofen in pharmaceutical preparations by spectrophotometric and liquid chromatographic methods.

A binary mixture of hyoscine butylbromide and ketoprofen was determined by 4 different methods. The first involved determination of hyoscine butylbromide and ketoprofen using the ratio-spectra first-derivative spectrophotometric technique at 211 and 234 nm over the concentration ranges of 2-14 and 5-45 microg/mL with mean accuracies 99.84 +/-0.

Spectrophotometric study of etodolac complexes with copper (II) and iron (III).

A rapid, simple, and selective method was developed for the determination of etodolac. The method depends on complexation of etodolac with copper (II) acetate and iron (III) chloride followed by extraction of complexes with dichloromethane and then measuring the extracted complexes spectrophotometrically at 684 and 385 nm in case of Cu (II) or Fe (III), respectively. Different factors affecting the reaction, such as pH, reagent concentration, and time, were studied.

Application of new membrane selective electrodes for the determination of drotaverine hydrochloride in tablets and plasma.

The construction and electrochemical response characteristics of poly vinyl chloride (PVC) membrane sensors for the determination of drotaverine hydrochloride were described. The sensors are based on the use of the ion association complexes of drotaverine cation with sodium phosphotungestate (Dro-PTA) or ammonium reineckate (Dro-R) counter anions as ion exchange sites in the PVC matrix. The performance characteristics of these sensors, which were evaluated according to IUPAC recommendations, reveal a fast, stable and linear response for drotaverine over the concentration range 10(-5) to 10(-2) M with cationic slopes of 49.

Simultaneous high-performance liquid chromatographic assay of furosemide and propranolol HCL and its application in a pharmacokinetic study.

A practical, sensitive, selective and efficient reversed-phase high-performance liquid chromatographic (HPLC) method is reported for the determination of two commonly used antihypertensive drugs, furosemide and propranolol hydrochloride. The drugs were eluted through a Nucleosil C(18) column with a mobile phase composed of 0.02 M potassium dihydrogen phosphate and acetonitrile (80:20, v/v) adjusted to pH 4.

Formulation and evaluation of sustained release floating capsules of nicardipine hydrochloride.

Nicardipine hydrochloride, a calcium channel blocker with significant vasodilating and antihypertensive activities, was formulated in this work as sustained release floating capsules. A hydrocolloid of high viscosity grade was used for the floating systems. The inclusion of sodium bicarbonate to allow evolution of CO2 to aid buoyancy was studied.

Stability-indicating spectrophotometric and densitometric methods for determination of aceclofenac.

Three methods were developed for the determination of aceclofenac in the presence of its degradation product, diclofenac. In the first method, third-derivative spectrophotometry (D3) is used. The D3 absorbance is measured at 283 nm where its hydrolytic degradation product diclofenac does not interfere.

Simultaneous determination of terbinafine HCL and triamcinolone acetonide by UV derivative spectrophotometry and spectrodensitometry.

A binary mixture of terbinafine hydrochloride and triamcinolone acetonide was determined by three different methods. The first one concerned with determination of both drugs using first derivative (D(1)) spectrophotometric technique at 297 and 274 nm over concentration ranges of 5-30 and 4-24 microg ml(-1), with mean percentage accuracies 99.90+/-0.

Investigation of ketoconazole copper(II) and cobalt(II) complexes and their spectrophotometric applications.

Ketoconazole, as a ligand, reacts quantitatively with copper(II) and cobalt(II) to form blue-colored, stable complexes in dichloromethane. These complexes can be spectrophotometrically measured at 720 and 612.5 nm in the case of Cu(II) or Co(II), respectively.

Bromometric determination of carbimazole.

Direct bromometric titration of carbimazole using N-bromosuccinimide (NBS) in the presence of methyl red as indicator and three indirect titrations using NBS, potassium bromate and dibromohydantoin (DBH) are developed. The direct procedure can be adopted for quantities of carbimazole ranging from 2-12 mg with mean accuracies of 100.2 +/- 0.