Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in Rats.

The role of the lungs and kidneys in liver regeneration after subtotal hepatectomy was studied on a rat model. It was found that production of hepatocyte growth factor (HGF) in the lungs and kidneys and expression of cytokine genes Il1b, Il6, Il10, and tnfa significantly increased. Analysis of the dynamics of lung macrophage population showed that accumulation of HGF and the increase in the expression of cytokine genes in the lungs were accompanied by simultaneous increase in the number of CD68 cells, which attested to the leading role of macrophages in activation of HGF synthesis in the lungs.

Role of Progenitor Cells in Liver Regeneration after Subtotal Resection.

In the liver of rats subjected to subtotal liver resection (80% organ weight), the expression of sox9 gene and SOX9 protein content increased and cells with hepatocyte morphology expressing SOX9 appeared; the proportion of cells expressing cytokeratin-19 also increased. Based on these data, we cannot completely exclude the involvement of resident progenitor cells and hepatocyte reprogramming in liver regeneration after subtotal resection, however, the contribution of these processes seems to be insignificant. The leading mechanism of liver mass recovery after subtotal resection is proliferation of hepatocytes.

Angiogenic Potential of Multipotent Stromal Cells from the Umbilical Cord: an In Vitro Study.

The mechanisms of proangiogenic activity of multipotent stromal cells from human umbilical cord were analyzed in vitro. The absence of secreted forms of proangiogenic growth factor VEGF-A in the culture medium conditioned by umbilical cord-derived multipotent stromal cells was shown by ELISA. However, the possibility of paracrine stimulation of cell proliferation, mobility, and directed migration of endothelial EA.

Effect of Endothelial Cells on Angiogenic Properties of Multipotent Stromal Cells from the Umbilical Cord during Angiogenesis Modeling in the Basement Membrane Matrix.

Short-term cell culturing on basement membrane matrix is a common and very convenient in vitro model of angiogenesis. We studied the possibility of interaction of multipotent stromal cells from the umbilical cord and Ea.hy926 endothelial cells on this model at the early and late periods of the experiment.

[EXPERIMENTAL MODELS OF STRESS URINARY INCONTINENCE].

Despite numerous medical and surgical treatment strategies available, the problem of stress urinary incontinence (SUI) in women is still not completely resolved. Continuing research is underway to modify the sling operations and develop new bulk-enhancing agents, including the use of tissue engineering and cell technologies. To evaluate the safety and effectiveness of new methods at the preclinical stage, adequate and reproducible experimental models of SUI in laboratory animals should be used.

Effect of multipotent stromal cells on the function of cell mitochondria in regenerating liver.

Intrasplenic allogeneic transplantation of multipotent stromal cells from the umbilical cord stimulates hepatocyte proliferation and promotes recovery of liver weight in rats after subtotal resection (80% organ weight). It can be hypothesized that this effect of multipotent stromal cells is due to more rapid recovery of the number of mitochondria and normalization of mitochondrial function of liver hepatocytes.

Hemopoietic cell proliferation and death in the regenerating fetal rat liver.

The proliferation and death of hemopoietic cells in the regenerating liver of 17-day outbred albino rat fetuses were studied during 2 days after resection of 20% of the organ. The mitotic index of hemopoietic cells in the resected liver increased significantly 24 h after the operation in comparison with the control fetuses. No increase in the counts of apoptotic hemopoietic cells was detected in the regenerating liver.

[Proliferation and cell death of hepatocytes in regenerating rat fetal liver].

Proliferation and death of hepatocytes in regenerating liver of 17-day white rat fetuses were investigated. During 2 days after liver resection (20%), animals were sacrificed every 3 h. In experimental groups, the index of Ki67-positive hepatocytes increased sharply in 15 h after liver resection.

[The effect of fraxiparine on the tomographic parameters of cerebral perfusion in young patients with cerebral vascular insufficiency].

Perfusion of the cerebral blood flow in patients with cerebral vascular insufficiency was studied using perfusion computed tomography before and after monotherapy with fraxiparine (single dosage 0.3 ml/2850 ME daily during 20 days) in 20 patients with clinical presentations of this disease in combination with antiphospholipid syndrome and the predominantly distinct hyper production of antiphospholipid antibodies revealed by ELISA. Fraxiparine was administered after the detection of significant changes (not less than 5%) of densitometric parameters characteristic of the state of brain tissue perfusion.

[Life expectency and fertility of postembrio stage drosophila after the pulse-periodic X-ray irradiation].

The effect of 13 Hz repetition rate X-ray pulses with 3 x 10(-6)-1.5 x 10(-4) Gr per pulse dose during 5 minute on drosophila's larvae and on pupae vas investigated. It was shown that the effect depends on drosophila's age as well as on X-ray dose and manifests itself in variation of life expectancy and fertility.

[Assessment of some metabolic parameters of white rats' liver after exposure to repetitive x-ray or microwave pulses].

Effects of repetitive X-ray and microwave pulses on the rat liver functions were investigated. The action of repetitive nanosecond X-ray is characterized by the metabolic dysfunction of the liver. In particular, it results in a considerable reduction in the ALT activity, augmentation of the AST/ALT ratio and decrease of the total protein content.

[Diagnostic value of radionuclide visualization of the reticuloendothelial system of macrophages in multiple sclerosis].

Scintigraphic examination of the liver, spleen, bone marrow and retroperitoneal lymph nodes with the help of colloidal radiopharmaceutical 113mIn was conducted in 55 patients with multiple sclerosis (MS) and in 13 patients with other diseases of the nervous system. The authors ascertained dysfunction of cells of the mononuclear phagocytic system (MPS) in the MS patients. Clinical manifestations of the disease were found to correlate with the findings of radionuclide imaging of the parenchymatous organs and lymph nodes.