Effects of the source of dietary protein on serum lower density lipoprotein (VLDL + LDL) and tocopherol levels in female rats.

The effect of dietary protein on lipid levels of serum and liver and mineral contents of bone tissue was studied in female rats by using 13 semi-purified diets differing with respect to protein source. The diets were characterized with regard to contents of protein, amino acids, fat, crude fiber, phytate, zinc, copper, iron, magnesium, calcium, sodium, potassium, tocopherol and metabolizable energy. Six of the proteins tested were of animal origin and six proteins used were plant proteins.

Determination of urinary cholic and chenodeoxycholic acid conjugates with radioimmunoassay.

Radioimmunoassays were developed for the determination of urinary unsulphated and total cholic and chenodeoxycholic acid conjugates. For the determination of unsulphated bile acid conjugates the urine was assayed directly after adjustment of pH to 7.4.

Bile acid synthesis and excretion following release of total extrahepatic cholestasis by percutaneous transhepatic drainage.

Urinary, biliary and serum bile acids were studied in three patients before and after percutaneous transhepatic drainage for total bile duct obstruction. Before drainage high urinary excretion of ten different bile acids occurred. The percentage distribution was: cholic and chenodeoxycholic acid (66-86%), hyocholic (3-16%), 3 beta, 12 alpha-dihydroxy-5-cholenoic (3-6%) and 3 beta-hydroxy-5-cholenoic acid (2-8%).

Excretion of bile acids in healthy children and children with cystic fibrosis.

Urinary bile acid excretion was investigated in twenty-two patients with cystic fibrosis (CF) and in seven healthy children. CF patients with and without antibiotic treatment were compared. Bile acids were determined in 24-h samples after separation into unconjugated, glycine conjugated, taurine conjugated and sulphate conjugated bile acids.

Levels of minerals, lipoproteins and lipids in plasma, liver and tibia from female rats fed on a zinc-supplemented rapeseed protein diet.

In order to study the effects of a zinc-supplemented dietary rapeseed protein concentrate on the contents of zinc and some other minerals in plasma, liver and bone tissue as well as on the levels of tocopherol, lipoproteins and lipids in plasma and total lipid contents of livers, groups of young female rats were fed for 4-week periods on semi-purified diets containing either rapeseed protein or casein as the sole source of protein at a 20% protein level. Despite normal levels of tissue zinc and plasma proteins, rats on diets and tocopherol in comparison to the casein-fed rats. Rats on rapeseed diet also seemed to have an increased lipid content of the liver possibly indicating a reduced hepatic synthesis or secretion of lipoproteins.

Urinary bile acid excretion in correlation to liver histopathology in cystic fibrosis.

Urinary bile acid excretion and liver morphology were compared in 25 patients with cystic fibrosis (CF). None showed clinical signs of liver disease. Most of the patients had normal liver function tests.

Evaluation of leg ulcer treatment with stereophotogrammetry. A pilot study.

This report describes stereophotogrammetric measurements of leg ulcers. A basic technical description of the method and its application in a pilot study are presented. The clinical evaluation of the healing of leg ulcers is compared to the photogrammetrically determined parameters--edge length, surface area, and volume--of the ulcers, and seems to fulfil the need for an objective method in the evaluation of leg ulcer treatment.

Zinc status and serum levels of retinol-binding protein, tocopherol and lower density lipoproteins in male and female rats fed on semi-purified diets containing rapeseed protein or casein.

Experiments were carried out in order to evaluate the effect of various levels of dietary zinc supplementation on the zinc status, growth and contents of retinol-binding protein (RBP), tocopherol and lower density lipoproteins (VLDL + LDL) in serum of rats fed on diets containing rapeseed protein concentrate (RPC) or casein as the sole source of protein. In male rats fed on RPC diet at a 10% protein 5% fat level, a highly significant correlation was found between dietary zinc content and the total tibia zinc content (r = 0.946) in the dietary zinc range of 35-137 microgram/mg.

Appearance of killer (K) cells in the mesenteric lymph nodes in Crohn's disease.

The local lymph node in Crohn's disease contains killer (K) cells which are able to lyse antibody-coated heterologous target cells. K cells are not found in mesenteric lymph nodes from control patients not suffering from Crohn's disease, whereas they are regularly found in similar amounts in the peripheral blood of both control and patients with Crohn's disease. Even mesenteric lymph node cells from areas not macroscopically affected by inflammatory disease show increased K cell activity as compared to control lymph node lymphocytes, suggesting a generalized mesenteric lymph node involvement with regard to K activity in Crohn's disease.

Effect of a dietary rapeseed protein concentrate on the contents of alpha-tocopherol and zinc in serum, liver and tibia of rats.

In order to study the effects of a dietary rapeseed protein concentrate (RPC) on tocopherol and zinc levels in serum, liver and tibia, female rats were fed a tocopherol-reduced diet for a 3-week period and were then transferred to either of a RPC diet or a control diet containing casein at 20% levels of protein. No unfavourable effects on food intakes or weight gains were recorded. The serum zinc content of the RPC group was slightly reduced and the serum tocopherol values significantly lowered in comparison to the control group.

Outcome of pregnancy from day 0 to 19 and serum tocopherol levels in mother rats fed on a rapeseed protein concentrate essentially free from glucosinolates.

A rapeseed protein concentrate containing only minute amounts of glucosinolates was fed to rats during gestation as the sole source of protein at a 20% dietary protein level. Compared with rats obtaining a casein-based diet at the same level of protein, the mother rats on the rapeseed diet showed much lower weight gains during the last trimester. At day 19 of gestation, rats treated with rapeseed protein showed a reduced number of live fetuses and an increased resorption quotient.

Scintigraphic examination of aneurysms of abdominal aorta and iliac arteries.

The use of a radionuclide (99Tcm-pertechnetate) for the diagnosis of aortic and iliac aneurysms is described. The method is rapid, harmless for the patient and is performed without puncture of arteries. The extent of the aneurysm and the perfusion of the kidneys can be visualized.

The contents of phytic acid in protein concentrates prepared from nigerseed, sunflower seed, rapeseed and poppy seed.

The contents of phytic acid were determined for protein-rich flours and protein concentrates prepared from nigerseed, sunflower seed, rapeseed and poppy seed. The values ranged from 6.89 to 8.

Effect of chlorogenic acid in a casein diet for rats. Nutritional and pathological observations.

3-week-old male rats were fed on a casein diet containing 1% (w/w) of chlorogenic acid in order to find out if the ingestion of chlorogenic acid may be associated with a reduced utilization of dietary proteins or with causing other adverse effects. The protein efficiency ratio, biological value and digestibility of this diet did not differ from a corresponding casein diet free from chlorogenic acid. No change was observed in the nitrogen balance of the experimental rats.