N-protein vaccine is effective against COVID-19: Phase 3, randomized, double-blind, placebo-controlled clinical trial.

Background: Despite the success of first-generation COVID-19 vaccines targeting the spike (S) protein, emerging SARS-CoV-2 variants have led to immune escape, reducing the efficacy of these vaccines. Additionally, some individuals are unable to mount an effective immune response to S protein-based vaccines. This has created a need for alternative vaccine strategies that are less susceptible to mutations and capable of providing broad and durable protection.

WES-based screening of 7,000 newborns: A pilot study in Russia.

The effective implementation of whole-exome sequencing- and whole-genome sequencing-based diagnostics in the management of children affected with genetic diseases and the rapid decrease in the cost of next-generation sequencing (NGS) enables the expansion of this method to newborn genetic screening programs. Such NGS-based screening greatly increases the number of diseases that can be detected compared to conventional newborn screening, as the latter is aimed at early detection of a limited number of inborn diseases. Moreover, genetic testing provides new possibilities for family members of the proband, as many variants responsible for adult-onset conditions are inherited from the parents.

Expanding phenotype of MED13-associated syndrome presenting novel de novo missense variant in a patient with multiple congenital anomalies.

Background: Whole exome sequencing allows rapid identification of causative single nucleotide variants and short insertions/deletions in children with congenital anomalies and/or intellectual disability, which aids in accurate diagnosis, prognosis, appropriate therapeutic interventions, and family counselling. Recently, de novo variants in the MED13 gene were described in patients with an intellectual developmental disorder that included global developmental delay, mild congenital heart anomalies, and hearing and vision problems in some patients.

Results: Here we describe an infant who carried a de novo p.

Assessing the stability-indicating properties of alternative potency assays for inactivated influenza vaccine.

Determination of the potency of a vaccine is critical to ensuring that an appropriate dose is delivered, lot-to-lot consistency is maintained, and that the formulation is stable over the life of the vaccine. The potency of inactivated influenza vaccines is determined routinely by the Single Radial Immunodiffusion (SRID) assay. A number of alternative potency assays have been proposed and have been under evaluation in recent years.

Cryopreservation of euploid blastocysts obtained after fertilization of in vitro matured ovarian tissue oocytes: a case report.

With the increased rate of stable remission after gonadotoxic cancer treatment, new methods of fertility preservation are required in order to provide the best possible care for oncological patients. Here, we report an original case of euploid blastocyst cryopreservation after in vitro maturation of ovarian tissue oocytes (OTO IVM). Thirty-three oocytes were obtained from the ovarian tissue after ovariectomy in the breast cancer patient.

[Comparative results of preimplantation genetic screening by array comparative genomic hybridization and new-generation sequencing].

Aneuploidies as quantitative chromosome abnormalities are a main cause of failed development of morphologically normal embryos, implantation failures, and early reproductive losses. Preimplantation genetic screening (PGS) allows a preselection of embryos with a normal karyotype, thus increasing the implantation rate and reducing the frequency of early pregnancy loss after IVF. Modern PGS technologies are based on a genome-wide analysis of the embryo.

Comparison of the results of preimplantation genetic screening obtained by a-CGH and NGS methods from the same embryos.

Chromosomal aneuploidies are known for being the main cause of abnormal development of embryos with normal morphology, their implantation failure and early reproductive losses in IVF treatments. Preimplantation genetic screening (PGS) allows selecting embryos with normal chromosomal content and increases IVF treatment efficiency due to higher implantation rates and less frequent early pregnancy losses. New technologies used for PGS allow making genome-wide analysis of the presence of all chromosomes in embryos.

Multicommutated stepwise injection determination of ascorbic acid in medicinal plants and food samples by capillary zone electrophoresis ultraviolet detection.

An automation of the extraction of analytes from solid samples into the aqueous phase based on multicommutated stepwise injection analysis concept has been suggested. The feasibility of the approach has been demonstrated by determination of ascorbic acid as model analyte. The method includes automated extraction of ascorbic acid from solid sample into borate buffer solution pH 8 in mixing chamber during vigorous mixing by nitrogen stream, and subsequent detection by capillary zone electrophoresis at 254 nm.

Rhythm analysis of orthogonal signals from human walking.

In physical terms, periodic movements of a human body resulting from walking produce a pulse sequence with repetition time T(1) (instant cadence frequency, 1/T(1)) and duration time T(2). Footstep forces generate periodic T(1) broadband seismic and sound signals due to the dynamic forces between the foot and the ground/floor with duration time T(2), which is equal to the time interval for a single footstep from heel strike to toe slap and weight transfer. In a human gait study (for normal speeds of walking), T(1) was detected as 0.

Extraction of the velocity of walking human's body segments using ultrasonic Doppler.

The focus of this paper is to experimentally extract the Doppler signatures of a walking human's individual body segments using an ultrasonic Doppler system (UDS) operating at 40 kHz. In a human's walk, the major contribution to Doppler velocities and acoustic scattering is from the foot, lower leg, thigh (upper leg) and torso. The Doppler signature of these human body segments are extracted experimentally.

[AlkB homologues in thermophilic bacteria of the genus Geobacillus].

Screening of alkane hydroxylase genes (alkB) was performed in the thermophilic aerobic bacteria of the genus Geobacillus. Total DNA was extracted from the biomass of 11 strains grown on the mixture of saturated C10-C20 hydrocarbons, PCR amplification of fragments of alkB genes was performed with degenerate oligonucleotide primers, PCR products were cloned and sequenced. For the first time in the genome of thermophilic bacteria the presence of a set of alkB gene homologues was revealed.

[Assessment of demographic and sociohygienic monitoring].

Experience in solving specific problems in sociohygienic monitoring shows that among risk factors, health losses are most commonly considered from the man-caused environmental load on the population, by employing statistical modeling methods and reports on monitoring socioeconomic risk factors are evidently insufficient. A cause-effect relation in the habitat-population health system should be revealed, by taking into account the combined multienvironmental influence of man-caused (chemical, physical) and social factors. For solution of the problems put in work, multivariate analysis was used to reveal the common mechanisms of an association of socioeconomic conditions, national composition, morbidity and disability rates, migration processes, and demographic structure with birth and mortality rates in villagers in the context of economic areas and municipal entities.

Human motion analyses using footstep ultrasound and Doppler ultrasound.

Human footsteps generate periodic broadband frequency envelopes of sound due to dynamic friction forces. Also, human body motion when walking is a cyclic temporal process. The individual body parts have different acoustic cross sections and velocities that form unique human Doppler signatures.

Ultrasonic wave generation due to human footsteps on the ground.

Human footsteps generate broadband frequency vibrations in the ground/floor and sound in the air from a few Hertz up to ultrasonic frequencies due to striking and sliding contacts between a foot and the ground/floor. The high-frequency (above 1 kHz) vibrations from footsteps were detected on a building floor, but were not detected on the outdoor ground, even at 1 m from a walker. This paper presents results of ultrasound registration from footsteps on the ground at greater distances.

Vibration and sound signatures of human footsteps in buildings.

The acoustic signature of a footstep is one of several signatures that can be exploited for human recognition. Early research showed the maximum value for the force of multiple footsteps to be in the frequency band of 1-4 Hz. This paper reports on the broadband frequency-dependent vibrations and sound pressure responses of human footsteps in buildings.

Nonlinear vibrations of buried landmines.

The seismo-acoustic method is one of the most promising emerging techniques for the detection of landmines. Numerous field tests have demonstrated that buried landmines manifest themselves at the surface through linear and nonlinear responses to acoustic/seismic excitation. The present paper describes modeling of the nonlinear response in the framework of the mass-spring model of the soil-mine system.

Nonlinear seismo-acoustic land mine detection and discrimination.

A novel technique for detection and discrimination of artificial objects, such as land mines, pipes, containers, etc., buried in the ground, has been developed and tested. The developed approach utilizes vibration (using seismic or airborne acoustic waves) of buried objects, remote measurements of soil surface vibration (using laser or microwave vibrometers), and processing of the measured vibration to extract mine's "vibration signatures.

[A method of genotyping clinical isolates of Ureaplasma urealyticum biovar Parvo].

A new method for typing clinical isolates of U. urealyticum (Parvo biovar) is based on SSCP analysis of amplicons of mba gene 5' region and upstream region. The mba gene is coding for MB gene of U.

[Mathematical approach to the problem of early diagnosis in heart failure].

A tool set for early detection of heart failure (HF) and its criteria for mass population survey in the polyclinic setting was developed. A two-stage screening programme was carried out. At the first stage, patients with cardiac abnormalities were detected.

[Functional organization of the cortico-reticular system of the brain in response to physical loads].

An estimation was carried out by factor analysis method of informative value of alpha-like rhythm, EEG theta-rhythm, local cerebral blood filling and oxygen tension (pO2) in estimation of functional state of cerebral structures under submaximal physical loads. Experiments were carried out on 35 rabbits with electrodes chronically implanted in the sensorimotor cortex and reticular formation. The obtained values were processed by a variant of factor analysis--a method of main components.