Antiviral Potential of Extracts (Prepared by Repercolation) Against Influenza A (H1N1) Virus.

An antiviral effect of extracts prepared from aerial parts of nine species and from leaves of two species of the genus L. was investigated for potential antiviral activity toward influenza A (H1N1) virus. The toxicity of dry extracts was analyzed, and the most selective extract was identified in vitro.

Combination of 3--Levulinoyl and 6--Trifluorobenzoyl Groups Ensures α-Selectivity in Glucosylations: Synthesis of the Oligosaccharides Related to α-(1 → 3)-d-Glucan.

Stereospecific α-glucosylation of primary and secondary OH-group at carbohydrate acceptors is achieved using glucosyl -phenyl-trifluoroacetimidate (PTFAI) donor protected with an electron-withdrawing 2,4,5-trifluorobenzoyl (TFB) group at O-6 and the participating levulinoyl (Lev) group at O-3. New factors have been revealed that might explain α-stereoselectivity in the case of TFB and pentafluorobenzoyl (PFB) groups at O-6. They are of conformational nature and confirmed by DFT calculations.

Mathematical modeling of the Fontan blood circulation supported with pediatric ventricular assist device.

The decompensated univentricular circulation is identified as one of the most challenging conditions and the application of the mechanical circulatory support (MCS) devices is proposed as therapeutic option for Fontan failure. Modelling methodologies are reported to identify the optimized types, extent and duration of required hemodynamic support using MCS. The specific parameters of device-body interaction during support of failing Fontan circulation within the design points of dedicated pediatric ventricular assist devices has not been previously defined.

Quality Control of Heparin Injections: Comparison of Four Established Methods.

Heparin is an anticoagulant medication that is usually injected subcutaneously. The quality of a set of commercial heparin injections from different producers was examined by NMR, IR, UV-Vis spectroscopies and potentiometric multisensor system. The type of raw material regarding heparin animal origin and producer, heparin molecular weight and activity values were derived based on the non-targeted analysis of H NMR fingerprints.

Distinguishing paracetamol formulations: Comparison of potentiometric "Electronic Tongue" with established analytical techniques.

Fast and inexpensive analytical tools for identification of the origin of pharmaceutical formulations are important to ensure consumers safety. This study explores the potential of potentiometric multisensor systems ("electronic tongues") in this type of application. 72 paracetamol samples purchased in different countries and produced by various companies were studied via infrared spectroscopy (IR), near infrared spectroscopy (NIR), nuclear magnetic resonance spectroscopy (NMR) and multisensor system (ET).

Properties of a recombinant GH49 family dextranase heterologously expressed in two recipient strains of Penicillium species.

The dexA gene encoding Penicillium funiculosum dextranase (GenBank accession MH581385) belonging to family 49 of glycoside hydrolases (GH49) was cloned and heterologously expressed in two recipient strains, P. canescens RN3-11-7 and P. verruculosum B1-537.

Complex effect of lignocellulosic biomass pretreatment with 1-butyl-3-methylimidazolium chloride ionic liquid on various aspects of ethanol and fumaric acid production by immobilized cells within SSF.

The pretreatment of softwood and hardwood samples (spruce and hornbeam wood) with 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) was undertaken for further simultaneous enzymatic saccharification of renewable non-food lignocellulosic biomass and microbial fermentation of obtained sugars to ethanol and fumaric acid. A multienzyme cocktail based on cellulases and yeast or fungus cells producing ethanol and fumaric acid were the main objects of [Bmim]Cl influence studies. A complex effect of lignocellulosic biomass pretreatment with [Bmim]Cl on various aspects of the process (both action of cellulases and microbial conversion of hydrolysates to target products) was revealed.

Assessment of bacterial resistance to organic solvents using a combined confocal laser scanning and atomic force microscopy (CLSM/AFM).

Using combined confocal laser scanning and atomic force microscopy (CLSM/AFM), bacterial viability under organic solvent stress was assessed at single cell level. Solvent-exposed bacteria stained with the LIVE/DEAD BacLight fluoresced green or red, allowing viable and dead cell discrimination. However, with toluene, butanol and acetonitrile, dually fluorescent cells appeared having compromised cell membranes.

Simultaneous species-specific PCR detection and viability testing of poly(vinyl alcohol) cryogel-entrapped Rhodococcus spp. after their exposure to petroleum hydrocarbons.

A method of simultaneous species-specific PCR detection and viability testing of poly(vinyl alcohol) cryogel-entrapped Rhodococcus spp. was developed that allowed the estimation of immobilized Rhodococcus opacus and Rhodococcus ruber survival after their exposure to petroleum hydrocarbon mixture. Spectrophotometric INT assay revealed high tolerance of gel-immobilized rhodococci to petroleum hydrocarbons, while among two Rhodococcus strains studied, R.

Selective adsorption of hydrocarbon-oxidizing Rhodococcus cells in a column with hydrophobized poly(acrylamide) cryogel.

A method for selective adsorption of Rhodococcus cells in the column with hydrophobized poly(acrylamide) cryogel (cryoPAAG) was developed that allowed rhodococci separation from mixed bacterial populations and their effective concentration within a sponge-like gel matrix. Hydrophobization of cryoPAAG using the n-dodecane graft (C12) was performed to enhance the adhesion of Rhodococcus cells to the cryogel; this was suggested by our finding that alkanotrophic rhodococci possess high adhesive activity (91-98%) towards n-alkanes, whereas other Gram-positive and Gram-negative bacteria tested did not adhere strongly to hydrocarbons. The selective index of the hydrophobic C12-cryoPAAG column for Rhodococcus cells was 72% that ensured their separation from complex bacterial cultures.