Objectives: To describe the laboratory assays used to investigate a suspected dengue case in summer 2012 in western Greece and to report the public health response.
Design Of Methods: Samples from the patient were tested for detection of Dengue virus (DENV) antibodies, NS1 antigen and RNA. Public health professionals interviewed residents of the village, and blood samples taken from 132 persons were tested for antibodies for DENV and West Nile virus (WNV).