Sudden death of a child associated with invasive non-typeable Haemophilus influenzae infection with underlying IgG subclass deficiency.

Haemophilus influenzae can be divided into typeable and non-typeable strains. Although non-typeable Haemophilus influenzae (NTHi) is less likely to be a fatal bacterium, invasive NTHi infection has been reported to increase worldwide. This study presents a case of sudden death of a child with invasive NTHi infection and underlying immunoglobulin G2 (IgG) deficiency.

Detection of deletion/insertion polymorphism profiles from single human hair shafts.

Background: Hair is a frequently encountered biological evidence in personal identification. The amount of nuclear DNA that can be extracted from a single strand of rootless hair is most limited, making the detection of short tandem repeat (STR) polymorphisms difficult. To overcome these limitations, deletion/insertion polymorphisms (DIP) as a new type of genetic marker have shown their benefits in detecting low-copy-number DNA.

Evaluation and SNP typing of DNA from ultraviolet-irradiated human bloodstains using TaqMan assay.

When detecting DNA profiles from forensic materials, it is pivotal to know the extent of degradation and which DNA marker can be genotyped. Ultraviolet (UV) is one of the common external factors that causes DNA damage, through which, an attempt to reveal cardinal genetic information can be made. In this study, after irradiation with three different UV wavelengths, UV-damaged DNA in the bloodstains was analyzed with long and short TaqMan assays using real-time PCR.

Real-time PCR assay for the detection of picoplankton DNA distribution in the tissues of drowned rabbits.

The detection of plankton DNA is one of the important methods for the diagnosis of drowning from postmortem tissues. This study investigated the quantities of picoplankton (Cyanobacteria) DNA in the lung, liver, kidney tissues and blood of drowned and non-drowned rabbits, and the sensitivity of detection of picoplankton DNA by polymerase chain reaction (PCR) detect for the diagnosis of death from drowning. For this purpose, the DNA of the 16S ribosomal RNA gene of picoplankton was quantitatively assayed from the tissues of drowned and non-drowned rabbits immersed in water after death.

Fundamental characteristics of synthetic adsorbents intended for industrial chromatographic separations.

With the aim of obtaining comprehensive information on the selection of synthetic adsorbents for industrial applications, effect of pore and chemical structure of industrial-grade synthetic adsorbents on adsorption capacity of several pharmaceutical compounds was investigated. For relatively low molecular mass compounds, such as cephalexin, berberine chloride and tetracycline hydrochloride, surface area per unit volume of polystyrenic adsorbents dominated the equilibrium adsorption capacity. On the contrary, effect of pore size of the polystyrenic adsorbents on the equilibrium adsorption capacity was observed for relatively high molecular mass compounds, such as rifampicin, Vitamin B12 and insulin.

Use of synthetic adsorbents in preparative normal-phase liquid chromatography.

Normal-phase liquid chromatographic separation using polymeric synthetic adsorbents was investigated. The retention behavior of dialkyl phthalates under a hexane-isopropanol eluent system revealed that both polystyrenic and polymethacrylic adsorbents can be used for normal-phase liquid chromatography, and that the polymethacrylic adsorbent has stronger retentivity than the polystyrenic adsorbent. Applicability of these synthetic adsorbents, especially the polymethacrylic adsorbents, was verified by the separation of polyunsaturated fatty acids, schizandrin in Schisandra chinensis fruit, tocopherols and tocotrienols in vegetable oils using a 10-microm adsorbent packed into an HPLC column.