Rapid fluorescence techniques were tested for identifying bacterial contamination in papermaking chemicals like starch and resin-based sizes.
Fluorescent probes, especially LIVE/DEAD and SYBR Green, proved most effective in distinguishing viable from non-viable bacterial cells under microscopy and flow cytometry.
While fluorescence microscopy gave bacterial counts similar to traditional plate counts, flow cytometry often showed lower counts, with detection limits varying between the two methods.