Publications by authors named "Egle M A Siqueira"

This study identified major phenolic compounds of the tucum-do-cerrado (Bactris setosa) peel, as well as antioxidant activity and total phytochemical compound concentration of different extracts of the peel and pulp of this fruit. Phenolic compounds of the different extracts of tucum-do-cerrado peel were identified and quantified using a high-performance liquid chromatography system coupled to a diode array detector (DAD). Total phytochemical compound content was determined by spectrophotometric assays and the antioxidant activity by ferric reducing antioxidant power and β-carotene/linoleic assays.

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This study investigated the effect of tucum-do-cerrado consumption in the oxidative status of iron-supplemented rats. Four groups of rats were treated: Control (AIN-93G), Tuc (AIN-93G added of tucum-do-cerrado), Fe (AIN-93G iron-enriched), or TucFe (AIN-93G with tucum-do-cerrado and iron-enriched) diet, for 30 days. Iron-enriched diet increased serum, liver, spleen, and intestine iron levels; transferrin saturation; liver lipid oxidation; mRNA levels of hepatic Hamp and Bmp6, and Nrf2 in the intestine.

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Vitamin A modulates inflammatory status, iron metabolism and erythropoiesis. Given that these factors modulate the expression of the hormone hepcidin (Hamp), we investigated the effect of vitamin A deficiency on molecular biomarkers of iron metabolism, the inflammatory response and the erythropoietic system. Five groups of male Wistar rats were treated: control (AIN-93G), the vitamin A-deficient (VAD) diet, the iron-deficient (FeD) diet, the vitamin A- and iron-deficient (VAFeD) diet or the diet with 12 mg atRA/kg diet replacing all-trans-retinyl palmitate by all-trans retinoic acid (atRA).

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The present study evaluated the nutritious potential of 18 fruits, all native of the Brazilian Cerrado biome. Mineral contents were determined by inductively coupled plasma atomic emission; phytic acid and tannin contents by a colorimetric and titrimetric method, respectively; and the potential mineral bioavailability by the molar ratio of phytic acid/mineral. Baru nut showed the highest zinc, copper, iron, phosphorus and magnesium content, and, together with macaúba, showed also the highest calcium content and caloric value.

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Carotenoid bioavailability is influenced by various factors including the food matrix. The release of those molecules from the food matrix is the initial and most important step in the absorption process. The relative bioavailability of bocaiuva pulp beta-carotene in relation to pure beta-carotene was assayed by a hepatic retinol store, in vitamin A deficient Wistar rats.

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Aim: This study examined the ability of tropical vegetables to reduce oxidative stress induced by vitamin A deficiency.

Methods: Vitamin A-deficient male Wistar rats were divided into four groups which were treated for 30 days with different diets: AIN-93G vitamin A-deficient diet (DD), DD supplemented with pure beta-carotene (beta-D) and DD supplemented with malanga (Xanthosoma sagittifolium) (MD) or purslane (Portulaca oleracea) (PD) leaves as the only source of vitamin A. The thiobarbituric acid-reactive substances (TBARS), reduced (GSH) and oxidized (GSSG) glutathione, and antioxidant enzyme activities were determined in the heart and liver.

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