DNA Damage in Bone Marrow Cells Induced by Femtosecond and Nanosecond Ultraviolet Laser Pulses.

Objective: The purpose of this study was to investigate the possible genotoxic impact of new generation 205 nm femtosecond solid-state laser irradiation on the DNA of murine bone marrow cells in vitro, and to compare the DNA damage caused by both femtosecond and nanosecond UV laser pulses.

Background Data: Recent experiments of corneal stromal ablation in vitro and in vivo applying femtosecond UV pulses showed results comparable with or superior to those obtained using nanosecond UV lasers. However, the possible genotoxic effect of ultrashort laser pulses was not investigated.

High-speed photorefractive keratectomy with femtosecond ultraviolet pulses.

Femtosecond near-infrared lasers are widely used for a number of ophthalmic procedures, with flap cutting in the laser-assisted in situ keratomileusis (LASIK) surgery being the most frequent one. At the same time, lasers of this type, equipped with harmonic generators, have been shown to deliver enough ultraviolet (UV) power for the second stage of the LASIK procedure, the stromal ablation. However, the speed of the ablation reported so far was well below the currently accepted standards.

Corneal stromal ablation with femtosecond ultraviolet pulses in rabbits.

Purpose: To determine the effectiveness of femtosecond ultraviolet (UV) pulses in ablating corneal stroma in a rabbit model and to compare the healing response between eyes treated with femtosecond UV pulses and eyes treated with standard excimer photorefractive keratectomy.

Setting: Laser Research Center, Vilnius University, Vilnius, Lithuania.

Design: Experimental study.

DNA damage in bone marrow cells induced by ultraviolet femtosecond laser irradiation.

Background Data: Research on the damaging effect of ultraviolet (UV) laser irradiation on the DNA of live organisms is still scarce, although UV lasers are increasingly being used in therapeutics and surgical treatment.

Objective: In this study we investigated the effect of new-generation 205-nm femtosecond solid-state laser irradiation on the DNA of murine bone marrow cells in vitro.

Materials And Methods: Mouse bone marrow cells in distinct plates were exposed to different doses of 205-nm femtosecond laser irradiation.

Corneal shaping and ablation of transparent media by femtosecond pulses in deep ultraviolet range.

Purpose: To assess the performance of a newly developed solid-state femtosecond ultraviolet (UV) laser system in corneal ablation.

Setting: Vilnius University, Laser Research Centre, Vilnius, Lithuania.

Methods: Femtosecond pulses in the deep UV range (205 nm) were obtained by the generation of the fifth-harmonic of an amplified Yb:KGW laser system (fundamental output at 1027 nm).