Publications by authors named "Ee Mei Teh"
Article Synopsis
- During normal metaphase in yeast, chromosomes are properly aligned by microtubules and cohesins, which help ensure sister chromatids separate accurately.
- A mutant strain, cdc15-2, displays chromosome mis-segregation upon recovering from a checkpoint activation despite being able to activate the spindle assembly checkpoint when exposed to disruption.
- The mis-segregation in the cdc15-2 mutant is attributed to premature spindle elongation and inadequate levels of Pds1p, indicating that slower cell cycle transitions can hinder effective checkpoint recovery.
In budding yeast, the secretory pathway is constitutively transporting cargoes such as invertase and alpha-factor throughout the cell division cycle. However, chitin synthase 2 (Chs2p), another cargo of the secretory pathway, is retained at the endoplasmic reticulum (ER) during mitosis when the mitotic kinase activity is high. Chs2p is exported from the ER to the mother-daughter neck only upon mitotic kinase destruction, indicating that the mitotic kinase activity is critical for the ER retention of Chs2p.
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