Publications by authors named "Eduardo S Moreno Prieto"
Article Synopsis
- Only one enzyme from family GH119, the α-amylase from Bacillus circulans, has been experimentally studied, highlighting the need for further research on this enzyme family.
- This study characterizes five enzymes from GH119, all of which are specific to α-amylase but produce different product profiles, indicating diversity within the family.
- The research provides the first kinetic constants for GH119 enzymes and validates previously predicted catalytic residues, suggesting that GH119 and GH57 should be classified together in a new clan (GH-T) in the CAZy database.
We report here the identification, characterization and three-dimensional (3D) structure determination of NaNga, a newly identified β-N-acetylgalactosaminidase from the Gram-negative soil bacterium Niabella aurantiaca DSM 17617. When recombinantly expressed in Escherichia coli, the enzyme selectively cleaved 4-nitrophenyl-N-acetyl-β-d-galactosamine (pNP-β-d-GalpNAc). The X-ray crystal structure of the protein was refined to 2.
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