Publications by authors named "Eduardo Miranda-Ulloa"

Undiagnosed HIV cases contribute to the increase in new infections, therefore estimating this figure is important in order to assess strategies in HIV control programs. The objective of this study was to estimate the number of undiagnosed HIV cases in the Cajamarca region in Peru between 2015 and 2021. HIV cases were obtained from three sources of information: The Regional Health Strategy for HIV Prevention and Control (ESPC-HIV); the HIV epidemiological notification application (Noti-HIV), and the laboratory information system (Netlab).

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As Peru is endemic to HTLV-1, it is important to know the reliability of the most used screening trademarks in our country. In the present study, we evaluated two brands of ELISA (Biokit and Wantai) for the detection of antibodies against HTLV-1/2 against Peruvian samples. Both brands were evaluated against 242 sera: 123 positive for HTLV-1 and 119 negative for HTLV-1 (Reference: Immunoblot).

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The objective of the study was to determine the diagnostic yield of the indirect immunofluorescence (IFI) test for the detection of antibodies against HTLV-1. A diagnostic test evaluation study was performed. HTLV-1-infected MT2 cells and HTLV-1-uninfected K-562 cells were cultured; then these cells were impregnated and fixed in sheets for immunofluorescence and faced to Peruvian sera.

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Objectives: Evaluate the efficiency of the enzyme-linked immunoelectrotransfer blot test (EITB) for the detection of human cysticercosis using purified native antigen mix from cysticercus fluid of Taenia solium.

Material And Methods: Observational study of the evaluation of the diagnostic test. The purified native antigen mix was extracted from the fluid of cysticercus from naturally parasitized pigs from areas considered endemic.

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In order to evaluate dot blot tests and latex agglutination for the detection of human cysticercosis with liquid antigen of Taenia solium cysticerci, 125 human sera were used, of which 60 were from people with cysticercosis confirmed by Western Blot, 45 with other parasitic diseases and 20 apparently healthy. The optimal concentration of antigen to impregnate dot blot strips was 0.01 ug/uL, and to impregnate the latex particles was 0.

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