Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes.

The quantitative analysis of cardiomyocyte function is essential for stem cell-based approaches for the in vitro study of human cardiac physiology and pathophysiology. We present a method to comprehensively assess the function of single human pluripotent stem cell-derived cardiomyocyte (hPSC-CMs) through simultaneous quantitative analysis of contraction kinetics, force generation, and electrical activity. We demonstrate that statistical analysis of movies of contracting hPSC-CMs can be used to quantify changes in cellular morphology over time and compute contractile kinetics.

Number and location of zero-group-velocity modes.

The frequency-wavenumber spectra of laminated media often exhibit anomalous modes with descending branches whose group velocity is negative, and these terminate at a minimum point at which the group velocity transitions from negative to positive. These minima are associated with resonant conditions in the medium, which have been clearly observed in experiments and in the nondestructive testing of laminated plates. Starting from first principles, this paper provides a theoretical analysis on the number and location of such zero-group-velocity (ZGV) modes for horizontally layered media bounded by any arbitrary combination of external boundaries.