Dietary factors in lipofuscinogenesis and ceroidogenesis.

The presence of ceroid pigments in human and animal tissues is associated with numerous pathological conditions in which the main pathogenic factor is the primary or secondary deficiency of vitamin E or imbalances between anti- and pro-oxidants. That oxidative stress, particularly through its consequent lipid peroxidation, plays a capital role in the genesis of ceroid pigments, is supported by numerous in vitro and in vivo studies. Discussed in this presentation are two examples of oxidative stress on ceroidogenesis, namely the in vivo rat model of dietary hepatic necrosis, and the in vitro formation of ceroid pigments by the aerobic incubation of unsaturated fat and blood cells.

Sequential histochemical studies of neuronal lipofuscin in human cerebral cortex from the first to the ninth decade of life.

The typical and most consistent physico-histochemical properties of lipofuscin granules, such as autofluorescence, sudanophilia, acid-fastness, PAS-reactivity, and lectin reactivities for diverse saccharide moieties have been generally detected in tissue specimens of old humans and animals. The purpose of this study was, therefore, to explore possible sequential variations of each of these properties in cortical neurons of the left cerebral temporo-parietal areas from individuals dying from the first to the ninth decade. Autofluorescence was studied with an ad hoc equipped microscope, sudanophilia was evaluated by Oil-red-O (ORO) staining, acid-fastness by long Ziehl-Nielsen reagent, PAS reactivity by the periodic-acid-Schiff reagent before and after diastase treatment, and the saccharide moieties by the use of a commercial kit of seven different biotinylated lectins.

Differential lectin histochemical studies on lipofuscin (age-pigment) and on selected ceroid pigments.

The persistent indiscriminate use of the term lipofuscin for the pigments encountered in pathological conditions, and which should be most properly termed ceroid pigments, is still creating unnecessary conceptual and nomenclature problems, and a great deal of confusion. While both the age-dependent lipofuscin and the pathologically formed ceroid pigments have somewhat similar physical and histochemical properties, sufficient differences to properly identify these two types of pigments are presented in this communication. In addition, because little is known on the saccharide components of lipofuscin and ceroid pigments in situ, we have in recent years explored the lectin binding characteristics of lipofuscin in human and rats, as well as in diverse ceroid pigments experimentally induced in rats.

Pigments in aging: an overview.

Although during the normal aging process there are numerous pigmentary changes, the best recognized are those of melanin and lipofuscin. Melanin may increase (e.g.