Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18-25 °C) for up to 48 h post mortem.

This study investigates the effect of time of storage of epididymides at room temperature and the addition of 20% of seminal plasma to the cryopreservation extender, on post thaw quality and fertility of ovine spermatozoa collected from the cauda epididymis. Spermatic kinetics, integrity and the stability of plasma membrane, damage to the acrosome and fertility following laparoscopic artificial insemination were evaluated in samples collected in an artificial vagina (AV) and from epididymides stored at room temperature for zero (G0), six (G6), twelve (G12), twenty-four (G24) and forty-eight (G48) hours post mortem. There were no significant differences in spermatic parameters between the methods of sample collection, except for progressive motility and velocity according to the straight path(VSL).