Comprehensive Genome Engineering Toolbox for Microalgae Based on CRISPR-Cas Systems.

Microalgae can produce industrially relevant metabolites using atmospheric CO and sunlight as carbon and energy sources, respectively. Developing molecular tools for high-throughput genome engineering could accelerate the generation of tailored strains with improved traits. To this end, we developed a genome editing strategy based on Cas12a ribonucleoproteins (RNPs) and homology-directed repair (HDR) to generate scarless and markerless mutants of the microalga .