Toward Marker-Assisted Selection in Breeding for Wilt Tropical Race-4 Type Resistant Bananas.

wilt is a soil borne fungal disease that has devastated banana production in plantations around the world. Most Cavendish-type bananas are susceptible to strains of f. sp.

Construction and Validation of CRISPR/Cas Vectors for Editing the Gene in Banana ( spp.).

Bananas and plantains are important staple food crops affected by biotic and abiotic stresses. The gene editing technique via Clustered Regularly Interspaced Short Palindromic Repeats associated with the Cas protein (CRISPR/Cas) has been used as an important tool for development of cultivars with high tolerance to stresses. This study sought to develop a protocol for the construction of vectors for gene knockout.

Expression of Genes Involved in Banana ( spp.) Response to Black Sigatoka.

This work aimed to evaluate the relative gene expression of the candidate genes , , , , and involved in the defense response to Black Sigatoka in banana cultivars Calcutta-4, Krasan Saichon, Grand Nain, and Akondro Mainty, by a quantitative real-time PCR. Biotic stress was imposed on 6-month-old plants during five sampling intervals under greenhouse conditions. The and genes were upregulated for the Calcutta-4- and Krasan Saichon-resistant cultivars, and were validated in this study.

Calcium-Binding Protein and Polymorphism in spp. Somaclones Resistant to .

The fresh fruits of 'Grande Naine' (Cavendish AAA- spp.) dominate the world market, especially in countries with a population in a situation of social vulnerability. However, Fusarium wilt, caused by the fungus f.

Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.

The objective of this systematic review (SR) was to select studies on the use of gene editing by CRISPR technology related to plant resistance to biotic stresses. We sought to evaluate articles deposited in six electronic databases, using pre-defined inclusion and exclusion criteria. This SR demonstrates that countries such as China and the United States of America stand out in studies with CRISPR/Cas.

Genetic diversity and population structure in banana (Musa spp.) breeding germplasm.

Bananas (Musa spp.) are one of the most highly consumed fruits globally, grown in the tropical and sub-tropical regions. We evaluated 856 Musa accessions from the breeding programs of the International Institute of Tropical Agriculture of Nigeria, Tanzania, and Uganda; the National Agricultural Research Organization of Uganda; the Brazilian Agricultural Research Corporation (Embrapa); and the National Research Centre for Banana of India.

Phytoparasitic Nematodes of spp. with Emphasis on Sources of Genetic Resistance: A Systematic Review.

Bananas are a staple food that considerably contributes to both food security and income generation, especially in countries of Africa, Asia, and Central and South America. The banana plant ( spp.) is affected by various pathogens, of main concern being the plant-parasitic nematodes associated with the rhizosphere, the most important of which are (burrowing nematode), sp.

Gene Editing for Plant Resistance to Abiotic Factors: A Systematic Review.

Agricultural crops are exposed to various abiotic stresses, such as salinity, water deficits, temperature extremes, floods, radiation, and metal toxicity. To overcome these challenges, breeding programs seek to improve methods and techniques. Gene editing by Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR/Cas-is a versatile tool for editing in all layers of the central dogma with focus on the development of cultivars of plants resistant or tolerant to multiple biotic or abiotic stresses.

Transcriptome Profiling of the Resistance Response of subsp. , var. Calcutta 4 to .

Banana ( spp.), which is one of the world's most popular and most traded fruits, is highly susceptible to pests and diseases. , responsible for Sigatoka leaf spot disease, is a principal fungal pathogen of spp.

Molecular, Histological and Histochemical Responses of Banana Cultivars Challenged with f. sp. with Different Levels of Virulence.

Fusarium wilt caused by f. sp. (Foc) is the most limiting factor in the banana agribusiness worldwide.

Gene Expression, Histology and Histochemistry in the Interaction between sp. and .

Bananas are the main fruits responsible for feeding more than 500 million people in tropical and subtropical countries. Black Sigatoka, caused by the fungus , is one of the most destructive disease for the crop. This fungus is mainly controlled with the use of fungicides; however, in addition to being harmful to human health, they are associated with a high cost.

Morphoanatomy and Histochemistry of Septal Nectaries Related to Female Fertility in Banana Plants of the 'Cavendish' Subgroup.

The objective of this study was to gain a deeper understanding of the morphoanatomical and histochemical structures that compose the nectary of pistillate flowers (female), which are involved in the female fertility of banana plants belonging to the 'Cavendish' subgroup. The diploid Calcutta 4 and the Grand Naine cultivar were used for the assessment. Five stages of floral development were proposed.

Phenolic Compounds and Oxidative Enzymes Involved in Female Fertility in Banana Plants of the Cavendish Subgroup.

The present study investigated phenolic compounds and enzymes involved in female fertility in banana plants of the Cavendish subgroup. The wild diploid Calcutta 4 and commercial cultivar Grand Naine (Cavendish subgroup) were used. The following five stages of floral development were proposed: S1 (partial vertical emission), S2 (total vertical emission), S3 (total horizontal emission), S4 (pre-anthesis), and S5 (anthesis).

Pre-selection of banana somaclones resistant to f. sp. , subtropical race 4.

- Fusarium wilt, caused by f. sp. (Foc), is one of the most destructive diseases affecting banana crops worldwide.

Genetic Improvement for Resistance to Black Sigatoka in Bananas: A Systematic Review.

Bananas are an important staple food crop in tropical and subtropical regions in Asia, sub-Saharan Africa, and Central and South America. The plant is affected by numerous diseases, with the fungal leaf disease black Sigatoka, caused by Morelet [anamorph: (Morelet) Deighton], considered one of the most economically important phytosanitary problem. Although the development of resistant cultivars is recognized as most effective method for long term control of the disease, the majority of today's cultivars are susceptible.

Improvements in the Resistance of the Banana Species to Fusarium Wilt: A Systematic Review of Methods and Perspectives.

The fungus f. sp. (FOC), tropical race 4 (TR4), causes wilt of banana, a pandemic that has threatened the cultivation and export trade of this fruit.

Genetic Diversity and Azole Fungicide Sensitivity in Field Populations in Brazil.

, causal agent of Sigatoka leaf spot, or yellow Sigatoka disease, is considered a major pathogen of banana ( spp.). Widely disseminated in Brazil, this study explored the genetic diversity in field populations of the pathogen from production areas in the Distrito Federal and the States of Bahia, Minas Gerais, and Rio Grande do Norte.

Bioactive amines changes during the ripening and thermal processes of bananas and plantains.

Bioactive amines are found in food and can be relevant for the assessment of fruits shelf life and nutritional quality. The pulp and peel of 20 banana and plantain were analyzed and the bioactive amine content varied according to the genotype, ripening stage, fruit tissue and thermal processing. In most of the analyzed genotypes, tyramine, histamine, dopamine, serotonin, spermidine, and spermine were decreased during the ripening process in the pulps.

Water stress in Musa spp.: A systematic review.

Background: The cultivation of bananas and other plants is limited by environmental stresses caused by climate change. In order to recognize physiological, biochemical and molecular components indicated to confer tolerance to water stress in Musa spp. we present the first systematic review on the topic.

Gene expression analysis in Musa acuminata during compatible interactions with Meloidogyne incognita.

Background And Aims: Endoparasitic root-knot nematodes (RKNs) ( Meloidogyne spp.) cause considerable losses in banana ( Musa spp.), with Meloidogyne incognita a predominant species in Cavendish sub-group bananas.

Characterisation of metabolic profile of banana genotypes, aiming at biofortified Musa spp. cultivars.

The banana is an important, widely consumed fruit, especially in areas of rampant undernutrition. Twenty-nine samples were analysed, including 9 diploids, 13 triploids and 7 tetraploids, in the Active Germplasm Bank, at Embrapa Cassava & Fruits, to evaluate the bioactive compounds. The results of this study reveal the presence of a diversity of bioactive compounds, e.

Genetic diversity and population structure of Musa accessions in ex situ conservation.

Background: Banana cultivars are mostly derived from hybridization between wild diploid subspecies of Musa acuminata (A genome) and M. balbisiana (B genome), and they exhibit various levels of ploidy and genomic constitution. The Embrapa ex situ Musa collection contains over 220 accessions, of which only a few have been genetically characterized.

Analysis of the leaf transcriptome of Musa acuminata during interaction with Mycosphaerella musicola: gene assembly, annotation and marker development.

Background: Although banana (Musa sp.) is an important edible crop, contributing towards poverty alleviation and food security, limited transcriptome datasets are available for use in accelerated molecular-based breeding in this genus. 454 GS-FLX Titanium technology was employed to determine the sequence of gene transcripts in genotypes of Musa acuminata ssp.