Testing and optimizing metabarcoding of iDNA from dung beetles to sample mammals in the hyperdiverse Neotropics.

Over the past few years, insects have been used as samplers of vertebrate diversity by assessing the ingested-derived DNA (iDNA), and dung beetles have been shown to be a good mammal sampler given their broad feeding preference, wide distribution and easy sampling. Here, we tested and optimized the use of iDNA from dung beetles to assess the mammal community by evaluating if some biological and methodological aspects affect the use of dung beetles as mammal species samplers. We collected 403 dung beetles from 60 pitfall traps.

Dung beetles from two sustainable-use protected forests in the Brazilian Amazon.

Background: The Amazon Forest is one of the world's most biodiverse ecosystems and yet its protected areas are understudied concerning insects and other invertebrates. These organisms are essential for tropical forests due to their ecological processes, with some species being very sensitive to habitat disturbances. Dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae) have been used as bioindicators for more than 30 years and were surveyed to assess the insect biodiversity of two sustainable-use forest reserves in the Brazilian Amazon.