Direct Measurement of Nucleoside Ribohydrolase Enzyme Activities in Cells Using F and C-Edited H NMR Spectroscopy.

Trichomoniasis is the most common nonviral sexually transmitted infection, affecting an estimated 275 million people worldwide. The causative agent is the parasitic protozoan . Although the disease itself is typically mild, individuals with trichomonal infections have a higher susceptibility to more serious conditions.

Structure-Guided Insight into the Specificity and Mechanism of a Parasitic Nucleoside Hydrolase.

is the causative parasitic protozoan of the disease trichomoniasis, the most prevalent, nonviral sexually transmitted disease in the world. is a parasite that scavenges nucleosides from the host organism via catalysis by nucleoside hydrolase (NH) enzymes to yield purine and pyrimidine bases. One of the four NH enzymes identified within the genome of displays unique specificity toward purine nucleosides, adenosine and guanosine, but not inosine, and atypically shares greater sequence similarity to the pyrimidine hydrolases.