Anoxic nitrification with carbon-based materials as terminal electron acceptors.

This study investigates the potential of humic substances (HS) and graphene oxide (GO), as extracellular electron acceptors (EEA) for nitrification, aiming to explore alternatives to sustain this process in wastewater treatment systems. Experimental results demonstrate the conversion of ammonium to nitrate (up to 87 % of conversion) coupled to the reduction of either HS or GO by anaerobic consortia. Electron balance confirmed the contribution of HS and GO to ammonium oxidation.

Impact of seed priming with Selenium nanoparticles on germination and seedlings growth of tomato.

Poor germination and seedlings growth can lead to significant economic losses for farmers, therefore, sustainable agricultural strategies to improve germination and early growth of crops are urgently needed. The objective of this work was to evaluate selenium nanoparticles (Se NPs) as nanopriming agents for tomato (Solanum lycopersicum) seeds germinated without stress conditions in both trays and Petri dishes. Germination quality, seedlings growth, synergism-antagonism of Se with other elements, and fate of Se NPs, were determined as function of different Se NPs concentrations (1, 10 and 50 ppm).

Nitrogen loss in coastal sediments driven by anaerobic ammonium oxidation coupled to microbial reduction of Mn(IV)-oxide.

Coastal sediments play a central role in regulating the amount of land-derived reactive nitrogen (N) entering the ocean, and their importance becomes crucial in vulnerable ecosystems threatened by anthropogenic activities. Sedimentary denitrification has been identified as the main sink of N in marine environments, while anaerobic ammonium oxidation with nitrite (anammox) has also been pointed out as a key player in controlling the nitrogen pool in these locations. Collected evidence in the present work indicates that the microbial biota in coastal sediments from Baja California (northwestern Mexico) has the potential to drive anaerobic ammonium oxidation linked to Mn(IV) reduction (manganammox).

Navigating the waters of nixtamalization: Sustainable solutions for maize-processing wastewater treatment.

Maize-processing wastewater, also known as nejayote, does represent a widespread residue originating from both small- and large-scale factories that produce maize-based products using the alkaline maize-cooking process (nixtamalization). Nejayote is a high-strength wastewater containing significant concentrations of soluble and insoluble organic and inorganic compounds resulting from the disintegration of maize, as well as from the substantial quantity of lime (Ca(OH)) used in the process. In order to make nixtamalization more sustainable in terms of water usage and to mitigate the health and environmental issues related to nejayote discharges into environmental matrices and public sewage systems, appropriate and effective treatment processes must be applied either before effluent disposal or for water reuse purposes.

Nitrate/nitrite-dependent anaerobic oxidation of methane (N-AOM) as a technology platform for greenhouse gas abatement in wastewater treatment plants: State-of-the-art and challenges.

Nitrate/nitrite-dependent anaerobic oxidation of methane (N-AOM) is a metabolic process recently discovered and partially characterized in terms of the microorganisms and pathways involved. The N-AOM process can be a powerful tool for mitigating the impacts of greenhouse gas emissions from wastewater treatment plants by coupling the reduction of nitrate or nitrite with the oxidation of residual dissolved methane. Besides specific anaerobic methanotrophs such as bacteria members of the phylum NC10 and archaea belonging to the lineage ANME-2d, recent reports suggested that other methane-oxidizing bacteria in syntrophy with denitrifiers can also perform the N-AOM process, which facilitates the application of this metabolic process for the oxidation of residual methane under realistic scenarios.

Novel biotechnologies for nitrogen removal and their coupling with gas emissions abatement in wastewater treatment facilities.

Wastewaters contaminated with nitrogenous pollutants, derived from anthropogenic activities, have exacerbated our ecosystems sparking environmental problems, such as eutrophication and acidification of water reservoirs, emission of greenhouse gases, death of aquatic organisms, among others. Wastewater treatment facilities (WWTF) combining nitrification and denitrification, and lately partial nitrification coupled to anaerobic ammonium oxidation (anammox), have traditionally been applied for the removal of nitrogen from wastewaters. The present work provides a comprehensive review of the recent biotechnologies developed in which nitrogen-removing processes are relevant for the treatment of both wastewaters and gas emissions.

Continuous anaerobic oxidation of methane: Impact of semi-continuous liquid operation and nitrate load on NO production and microbial community.

This work proves the feasibility of employing regular secondary activated sludge for the enrichment of a microbial community able to perform the anaerobic oxidation of methane coupled to nitrate reduction (N-AOM). After 96 days of activated sludge enrichment, a clear N-AOM activity was observed in the resulting microbial community. The methane removal potential of the enriched N-AOM culture was then studied in a stirred tank reactor (STR) operated in continuous mode for methane supply and semi-continuous mode for the liquid phase.

The role of humic substances in mitigating greenhouse gases emissions: Current knowledge and research gaps.

Humic substances (HS) constitute a highly transformed fraction of natural organic matter (NOM) with a heterogeneous structure, which is rich in electron-transferring functional moieties. Because of this feature, HS display a versatile reactivity with a diversity of environmentally relevant organic and inorganic compounds either by abiotic or microbial processes. Consequently, extensive research has been conducted related to the potential of HS to drive relevant processes in bio-engineered systems, as well as in the biogeochemical cycling of key elements in natural environments.

Stabilization of potentially toxic elements contained in mine waste: A microbiological approach for the environmental management of mine tailings.

Metals are key materials extensively employed in several industries to produce technological and daily-life products. The mining industry that produces such commodities generates Tons of waste that if not remediated can be transferred to the surrounding environment, thus representing a water, air, and soil pollution threat. In this work, we evaluated the feasibility of microbial sulfate reduction (SR) as a management strategy for this waste.

Humic Substances Mediate Anaerobic Methane Oxidation Linked to Nitrous Oxide Reduction in Wetland Sediments.

Humic substances are redox-active organic molecules, which play pivotal roles in several biogeochemical cycles due to their electron-transferring capacity involving multiple abiotic and microbial transformations. Based on the redox properties of humic substances, and the metabolic capabilities of microorganisms to reduce and oxidize them, we hypothesized that they could mediate the anaerobic oxidation of methane (AOM) coupled to the reduction of nitrous oxide (NO) in wetland sediments. This study provides several lines of evidence indicating the coupling between AOM and the reduction of NO through an extracellular electron transfer mechanism mediated by the redox active functional groups in humic substances (e.

Electron shuttling mediated by humic substances fuels anaerobic methane oxidation and carbon burial in wetland sediments.

Key pathways for the anaerobic oxidation of methane (AOM) have remained elusive, particularly in organic rich ecosystems. In this work, the occurrence of AOM driven by humus-catalyzed dissimilatory iron reduction was investigated in sediments from a coastal mangrove swamp. Anoxic sediment incubations supplied with both goethite (α-FeOOH) and leonardite (humic substances (HS)) displayed an average AOM rate of 10.

Anaerobic ammonium oxidation linked to sulfate and ferric iron reduction fuels nitrogen loss in marine sediments.

Availability of fixed nitrogen is a pivotal driver on primary productivity in the oceans, thus the identification of key processes triggering nitrogen losses from these ecosystems is of major importance as they affect ecosystems function and consequently global biogeochemical cycles. Denitrification and anaerobic ammonium oxidation coupled to nitrite reduction (Anammox) are the only identified marine sinks for fixed nitrogen. The present study provides evidence indicating that anaerobic ammonium oxidation coupled to the reduction of sulfate, the most abundant electron acceptor present in the oceans, prevails in marine sediments.

Anaerobic Methane Oxidation Driven by Microbial Reduction of Natural Organic Matter in a Tropical Wetland.

Wetlands constitute the main natural source of methane on Earth due to their high content of natural organic matter (NOM), but key drivers, such as electron acceptors, supporting methanotrophic activities in these habitats are poorly understood. We performed anoxic incubations using freshly collected sediment, along with water samples harvested from a tropical wetland, amended with C-methane (0.67 atm) to test the capacity of its microbial community to perform anaerobic oxidation of methane (AOM) linked to the reduction of the humic fraction of its NOM.