Publications by authors named "Edgar Del Llano"
Article Synopsis
- * Researchers created Huh7.5 ADAR1 knockout (KO) cell lines to explore ADAR1's function in liver cells and found that these cells experienced growth arrest and failed to recover after IFN treatment.
- * Analysis revealed significant changes in the transcriptome and translatome of the KO cells, particularly impacting RNA polymerase III transcription and small RNA levels, indicating ADAR1's vital role in small RNA metabolism and ribosome production.
Nucleic Acids Res
December 2023
Article Synopsis
- Translation is essential for development, particularly during the silenced transcription phase of oocytes and early embryos.
- A genome-wide translatome analysis revealed that while global protein synthesis decreases during M-phases, the initiation and elongation of translation are activated, showing a dynamic shift in how specific mRNAs are translated.
- This study provides new insights into gene expression regulation during oocyte meiosis and the first two embryonic mitoses, marking a significant step towards understanding the molecular mechanisms of translation control in early development.
Int J Mol Sci
February 2023
Article Synopsis
- Reciprocal translocation (RT) carriers have a higher risk of infertility, miscarriages, and children with health issues due to the production of unbalanced gametes.
- Prenatal diagnosis (PND) and preimplantation genetic diagnosis (PGD) can help minimize these risks, but there are concerns about the effectiveness of sperm fluorescence in situ hybridization (spermFISH) as a diagnostic tool for RT carriers.
- A study of 41 RT carriers indicated that acrocentric chromosomes lead to more unbalanced gametes, and the variability in balanced sperm rates suggests that using spermFISH routinely may not be beneficial for these patients.
Eur J Cell Biol
April 2022
Article Synopsis
- * The maturation-promoting factor (MPF), made up of CDK1 and Cyclin B1, is crucial for resuming meiosis, but the mechanisms behind its activation are not fully understood.
- * This study identifies SGK1, a specific kinase expressed in mouse oocytes, as a key player that influences CDK1 activation; inhibiting SGK1 delays meiotic progression, but introducing active CDK1 can reverse this effect, shedding light on how meiosis is regulated.
Article Synopsis
- Increasing maternal age in mammals leads to lower quality oocytes, higher aneuploidy rates, and reduced developmental ability.
- Research shows that many mRNAs are translated differently in oocytes from older females compared to younger ones, particularly affecting those related to the cell cycle.
- Specific proteins like CASTOR1 and SGK1 are linked to errors in chromosome alignment during meiosis, indicating that improper translation of proteins at the start of meiosis is a factor in the age-related decline in reproductive success.
Int J Mol Sci
February 2020
Article Synopsis
- Meiotic maturation of oocytes relies on maternal mRNA translation, requiring a new method called Scarce Sample Polysome Profiling (SSP-profiling) for better analysis of polysome-associated transcripts.
- This method combines sucrose gradient ultracentrifugation with qRT-PCR to quantify rRNAs and is effective for both small and large sample sizes.
- Using SSP-profiling, researchers explored the translatome of mouse oocytes, discovering 1847 transcripts related to important cellular functions and key steps in meiosis, including those with unknown roles that may contribute to oocyte aneuploidy.
Article Synopsis
- Culture media for assisted reproduction often include gonadotropin hormones to help mature oocytes, but the impact on protein synthesis is not fully clear.
- A study with mouse oocytes showed that the addition of follicle-stimulating hormone (FSH) reduced the incorporation of methionine, a key amino acid, into new proteins in both denuded oocytes and oocytes in cumulus-oocyte complexes.
- FSH was found to affect amino acid uptake through its receptor in oocytes, which may influence their overall metabolism and physiological functions without significantly impacting major translational regulators.
Int J Mol Sci
September 2018
Article Synopsis
- The study investigates how maternal age impacts chromosome segregation errors during meiosis I in mouse oocytes.
- Older female mice (12 months) show faster meiotic progression and altered nuclear lamina structures compared to younger females (2 months).
- Increased activity of MPF and higher translation of related factors in aged oocytes may lead to premature meiotic processes, contributing to chromosomal errors during meiosis I.
Article Synopsis
- Fully grown mammalian oocytes rely on RNA transcripts made earlier in development for their functions, regulated by RNA localization and translation mechanisms.
- In mouse oocytes, three cap-dependent translational repressors (4E-BP1, 4E-BP2, and 4E-BP3) are produced at the mRNA level, but only 4E-BP1 is active as a protein, promoting translation after nuclear envelope breakdown through phosphorylation.
- Key regulators of 4E-BP1 phosphorylation during meiosis are mTOR and CDK1 kinases, with evidence suggesting that this regulatory pathway is also present and likely functions similarly in human oocytes.
Article Synopsis
- The study investigates the role of EPHB6 in colorectal tumorigenesis, finding that its manipulation does not impact the growth or movement of colon cancer cells in various models.
- Using EphB6 knockout mice, researchers determined that the inactivation of EPHB6 does not efficiently initiate intestinal tumors or affect survival and tumor characteristics.
- However, introducing EPHB6 into colon cancer cells reduced lung metastasis, suggesting that the loss of EPHB6 is linked to increased metastatic spread in colorectal cancer.
