Effectiveness of Acetazolamide in Patients With Heart Failure: A Systematic Review and Meta-Analysis.

This systematic review and meta-analysis evaluated the efficacy and safety of acetazolamide as an adjunctive diuretic therapy in acute heart failure (AHF) patients. A comprehensive literature search was conducted across multiple electronic databases, including PubMed, Embase, Cochrane Library, and Scopus, identifying seven studies (five randomized controlled trials and two observational studies) that met the eligibility criteria. The analysis revealed that acetazolamide significantly enhanced mean natriuresis (mean differences (MD) 52.

Colorimetric and electrochemical analysis of DNAzyme-LAMP amplicons for the detection of Escherichia coli in food matrices.

Foodborne bacteria like Escherichia coli threaten global food security, necessitating affordable, on-site detection methods, especially in resource-limited settings. This study optimized loop-mediated isothermal amplification (LAMP) integrated with peroxidase-mimicking G-quadruplex DNA structures (DNAzyme), termed DNAzyme-LAMP which was designed to incorporate two different catalytic DNAzymes per amplification unit, enabling colorimetric detection of E. coli in leafy vegetables and milk samples.

Development of a simple prediction model for cowpea yield under environmentally growth-restricted conditions.

New and simple crop yield prediction methods are expected to be developed owing to the increasing environmental stress caused by climate change. Algorithms of machine learning could be a powerful tool for predicting crop yield; however, the required feature variables and differences in their prediction accuracy are poorly addressed. The objectives of this study were to identify the best combination of feature variables to predict the yield of cowpea (Vigna unguiculata), which is widely grown in central Sudan Savanna under environmentally restricted conditions, and clarify the differences in the accuracy of major machine learning algorithms.

Comparative Effectiveness of Bariatric Metabolic Surgery Versus Glucagon-Like Peptide-1 Receptor Agonists on Cardiovascular Outcomes and Mortality: A Meta-Analysis.

Cardiovascular disease (CVD) remains a leading cause of morbidity and mortality worldwide, particularly in individuals with obesity and type 2 diabetes mellitus (T2DM). This meta-analysis aimed to compare the effectiveness of bariatric metabolic surgery (BMS) and glucagon-like peptide-1 receptor agonists (GLP-1RAs) on cardiovascular outcomes and mortality in patients with obesity. A comprehensive literature search was conducted in Medline, Embase, and Cochrane CENTRAL from inception to September 15, 2024.

Initial development of a rapid, portable, stall-side ELISA for the measurement of equine adrenocorticotropic hormone.

Pituitary pars intermedia dysfunction (PPID) is a neurodegenerative disease of senior horses. Loss of dopaminergic inhibition of the melanotropes of the pars intermedia leads to increased concentrations of pro-opiomelanocortin (POMC)-derived peptides. Diagnosis is challenging due to pre-analytical variables, such as sample storage, handling, and time to analysis.

Partial privatization and cooperation in biofilms.

The evolution of cooperation in microbes is a challenge to explain because microbes producing costly goods for the benefit of any strain types (cooperators) often withstand the threat of elimination by interacting with individuals that exploit these benefits without contributing (defectors). Here we developed an individual-based model to investigate whether partial privatization via the partial secretion of goods can favor cooperation in structured, surface-attaching microbial populations, biofilms. Whether partial secretion can favor cooperation in biofilms is unclear for two reasons.

MORTALITY, SURVIVAL, AND SEROLOGIC RESULTS FOR ELK (CERVUS CANADENSIS) IN THE CUMBERLAND MOUNTAINS OF TENNESSEE, USA.

Comprehensive disease surveillance has not been conducted in elk (Cervus canadensis) in Tennessee, US, since their reintroduction to the state 20 yr ago. We identified causes of death, estimated annual survival, and identified pathogens of concern in elk at the North Cumberland Wildlife Management Area (NCWMA), Tennessee, US. In 2019 and 2020, we captured 29 elk (21 females, eight males) using chemical immobilization and fitted individuals with GPS collars with mortality sensors.

Long-term effect of pulmonary rehabilitation in idiopathic pulmonary fibrosis: a randomised controlled trial.

Background: Idiopathic pulmonary fibrosis (IPF) is characterised by worsening dyspnoea and exercise intolerance.

Research Question: Does a long-term pulmonary rehabilitation improve exercise tolerance in patients with IPF treated with standard antifibrotic drugs, which are expected to reduce disease progression?

Methods: This open-label randomised controlled trial was performed at 19 institutions. Stable patients receiving nintedanib were randomised into pulmonary rehabilitation and control groups (1:1).

Black Queen Hypothesis, partial privatization, and quorum sensing evolution.

Microorganisms produce costly cooperative goods whose benefit is partially shared with nonproducers, called 'mixed' goods. The Black Queen Hypothesis predicts that partial privatization has two major evolutionary implications. First, to favor strains producing several types of mixed goods over nonproducing strains.

An alternating current electrokinetics biosensor for rapid on-site serological screening of Taenia solium cysticercosis infection.

Cysticercosis, caused by Taenia solium infection, is a leading cause of acquired epilepsy in many developing countries. Several types of immunoassays have been developed for the detection of Taenia solium infection in both infected humans and livestock animals. However, these methods require central laboratory facilities and are both time- and labor-consuming with longer than desired turnaround time.

Optimization of ACEK-enhanced, PCB-based biosensor for highly sensitive and rapid detection of bisphenol a in low resource settings.

In this study, we developed a low-cost and easy-to-use capacitive biosensor employing printed-circuit-board (PCB)-based technique for electrode fabrication and a specific alternative current (AC) signal for AC Electrokinetics (ACEK) effect excitation. Fast, accurate, and highly sensitive detection and quantification of bisphenol A (BPA) was achieved. An easy characterization of the biofunctionalization process is introduced by measuring interfacial capacitance which is simple and superior to most of methods currently in use.

Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications.

A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnostics. Presently, most miRNA detection methods suffer from drawbacks, e.

Electrochemical Detection of Serum Antibodies Against Subspecies .

subsp. (MAP) causes a chronic inflammatory intestinal disease, called Johne's disease (JD) in many ruminants. In the dairy industry, JD is responsible for significant economic losses due to decreased milk production and premature culling of infected animals.

Species diversity and phylogeography of (Asian dogwood) captured by genomic and genic microsatellites.

(Asian dogwood), an East Asia native tree, is the most economically important species of the dogwood genus, owing to its desirable horticultural traits and ability to hybridize with North America-native dogwoods. To assess the species genetic diversity and to better inform the ongoing and future breeding efforts, we assembled an herbarium and arboretum collection of 131 noncultivated specimens. Genotyping and capillary electrophoresis analyses of our collection with the newly developed genic and published nuclear genomic microsatellites permitted assessment of genetic diversity and evolutionary history of the species.

An on-site, highly specific immunosensor for Escherichia coli detection in field milk samples from mastitis-affected dairy cattle.

Bovine mastitis is the most economically important infectious disease in dairy industry, and Escherichia coli (E. coli) is one of the major causative pathogens. Rapid identification and quantitative detection of E.

An artificial metalloenzyme biosensor can detect ethylene gas in fruits and Arabidopsis leaves.

Enzyme biosensors are useful tools that can monitor rapid changes in metabolite levels in real-time. However, current approaches are largely constrained to metabolites within a limited chemical space. With the rising development of artificial metalloenzymes (ArM), a unique opportunity exists to design biosensors from the ground-up for metabolites that are difficult to detect using current technologies.

Combined e-pharmacophore based screening and docking of PI3 kinase with potential inhibitors from a database of natural compounds.

Phospho inositide 3-kinase (PI3 K) is a promising target for the design of anticancer drugs and is of significant concern in developing selective isoforms as inhibitors for cancer treatments. The results obtained from the computational analysis were selected based on Glide score and drug binding interaction features. Molecular docking studies and prime MM-GBSA energy calculations showed STOCK1N-77648 with optimal binding features for further consideration.

Characterization of Ethanol Extracted Cell Wall Components of Subsp. .

Antigens extracted using ethanol (EtOH) and incorporated in the EtOH vortex ELISA (EVELISA) test have previously shown high specificity and sensitivity for detecting subspecies () and infections in cattle. The objective of this study is to define the components present in the EtOH extract. We show that this extract is composed of lipid, carbohydrate, and proteins on the surface of the bacilli, and that EtOH removes the outer layer structure of which comprise these elements.

Modelling Bovine Granuloma Formation In Vitro upon Infection with Subspecies .

subspecies () causes chronic granulomatous disease in cattle and ruminant livestock, causing substantial economic losses. Current vaccines delay clinical signs but cannot train the immune system to fully eradicate latent . During latency, uses host defenses, cage-like macrophage clusters called granuloma, as incubators for months or years.

Screening of caspase-3 inhibitors from natural molecule database using e-pharmacophore and docking studies.

Caspase a protease family member, have a vital role in cell death and inflammation process. Caspase-3, an effector caspase controls the regulation of apoptosis and has an anti apoptotic function. The mechanical significance of restoring apoptosis signaling to selectively target malignant cells is utilized to develop strong therapeutic strategies by the caspase family of mortality - induction molecules.

Identification of novel compound heterozygous mutations in ACO2 in a patient with progressive cerebral and cerebellar atrophy.

Background: The tricarboxylic acid (TCA) cycle is a sequence of catabolic reactions within the mitochondrial matrix, and is a central pathway for cellular energy metabolism. Genetic defects affecting the TCA cycle are known to cause severe multisystem disorders.

Methods: We performed whole exome sequencing of genomic DNA of a patient with progressive cerebellar and cerebral atrophy, hypotonia, ataxia, seizure disorder, developmental delay, ophthalmological abnormalities and hearing loss.

Ocular findings in Japanese children with Down syndrome: the course of visual acuity and refraction, and systemic and ocular anomalies.

Purpose: To investigate the age-related development of refractive errors and changes of visual acuity (VA), and the systemic and ocular anomalies in Japanese children and young adults with Down syndrome (DS).

Design: Retrospective cohort study.

Subjects And Methods: This study involved 222 Japanese children and young adults with DS (age range: 3 months to 19 years) seen at the Department of Ophthalmology, Shiga Medical Center for Children, Shiga, Japan.